Morphology and dynamics of clathrin/GGA1-coated carriers budding from the trans-Golgi network

被引:99
作者
Puertollano, R [1 ]
van der Wel, NN [1 ]
Greene, LE [1 ]
Eisenberg, E [1 ]
Peters, PJ [1 ]
Bonifacino, JS [1 ]
机构
[1] Netherlands Canc Inst, Div Tumor Biol, Amsterdam, Netherlands
关键词
D O I
10.1091/mbc.02-07-0109
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Sorting of transmembrane proteins and their ligands at various compartments of the endocytic and secretory pathways is mediated by selective incorporation into clathrin-coated intermediates. Previous morphological and biochemical studies have shown that these clathrin-coated intermediates consist of spherical vesicles with a diameter of 60-100 nm. Herein, we report the use of fluorescent imaging of live cells to demonstrate the existence of a different type of transport intermediate containing associated clathrin coats. Clathrin and the adaptors GGA1 and adaptor protein-1, labeled with different spectral variants of the green fluorescent protein, are shown to colocalize to the trans-Golgi network and to a population of vesicles and tubules budding from it. These intermediates are highly pleiomorphic and move toward the peripheral cytoplasm for distanced of up to 10 mum with average speeds of -1 mum/s. The labeled clathrin and GGA1 cycle on and off membranes with half-times of 10-20 s, independently of vesicle budding. Our observations indicate the existence of a novel type of trans-Golgi network-derived carriers containing associated clathrin, GGA1 and adaptor protein-1 that are larger than conventional clathrin-coated vesicles, and that undergo long-range translocation in the cytoplasm before losing their coats.
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收藏
页码:1545 / 1557
页数:13
相关论文
共 43 条
[1]   SEQUENTIAL COUPLING BETWEEN COPII AND COPI VESICLE COATS IN ENDOPLASMIC-RETICULUM TO GOLGI TRANSPORT [J].
ARIDOR, M ;
BANNYKH, SI ;
ROWE, T ;
BALCH, WE .
JOURNAL OF CELL BIOLOGY, 1995, 131 (04) :875-893
[2]  
Boman AL, 2001, J CELL SCI, V114, P3413
[3]   Biological basket weaving: Formation and function of clathrin-coated vesicles [J].
Brodsky, FM ;
Chen, CY ;
Knuehl, C ;
Towler, MC ;
Wakeham, DE .
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2001, 17 :517-568
[4]   Yeast Gga coat proteins function with clathrin in Golgi to endosome transport [J].
Costaguta, G ;
Stefan, CJ ;
Bensen, ES ;
Emr, SD ;
Payne, GS .
MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (06) :1885-1896
[5]   Spatially regulated recruitment of clathrin to the plasma membrane during capping and cell translocation [J].
Damer, CK ;
O'Halloran, TJ .
MOLECULAR BIOLOGY OF THE CELL, 2000, 11 (06) :2151-+
[6]   GGAs: A family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex [J].
Dell'Angelica, EC ;
Puertollano, R ;
Mullins, C ;
Aguilar, RC ;
Vargas, JD ;
Hartnell, LM ;
Bonifacino, JS .
JOURNAL OF CELL BIOLOGY, 2000, 149 (01) :81-93
[7]   Cooperation of GGAs and AP-1 in packaging MPRs at the trans-Golgi network [J].
Doray, B ;
Ghosh, P ;
Griffith, J ;
Geuze, HJ ;
Kornfeld, S .
SCIENCE, 2002, 297 (5587) :1700-1703
[8]   Interaction of the cation-dependent mannose 6-phosphate receptor with GGA proteins [J].
Doray, B ;
Bruns, K ;
Ghosh, P ;
Kornfeld, S .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (21) :18477-18482
[9]   Spatial control of coated-pit dynamics in living cells [J].
Gaidarov, I ;
Santini, F ;
Warren, RA ;
Keen, JH .
NATURE CELL BIOLOGY, 1999, 1 (01) :1-7
[10]   EXIT OF NEWLY SYNTHESIZED MEMBRANE-PROTEINS FROM THE TRANS CISTERNA OF THE GOLGI-COMPLEX TO THE PLASMA-MEMBRANE [J].
GRIFFITHS, G ;
PFEIFFER, S ;
SIMONS, K ;
MATLIN, K .
JOURNAL OF CELL BIOLOGY, 1985, 101 (03) :949-964