Cloning and expression of a distinct subclass of plant thioredoxins

被引：30

作者：

Juttner, J ^{[1
]}

Olde, D ^{[1
]}

Langridge, P ^{[1
]}

Baumann, U ^{[1
]}

机构：

[1] Univ Adelaide, Dept Plant Sci, Glen Osmond, SA 5064, Australia

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 2000年 / 267卷 / 24期

关键词：

Poaceae; pollen; reverse transcription PCR; thioredoxin; protein expression;

D O I：

10.1046/j.1432-1327.2000.01811.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

mRNAs encoding a novel thioredoxin were isolated from pollen RNA of Lolium perenne (LpTrx), Hordeum bulbosum (HbTrx), Phalaris coerulescens (PTrx) and Secale cereale (ScTrx). The cDNAs contain a single ORF of 393 bp encoding a protein of 131 amino acids. The predicted proteins showed highest homology to plant thioredoxins of the h class yet form a distinct subgroup that is characterized by a high level of sequence conservation (95.4-97.7% identity). GenBank searches revealed additional members of this subclass in tomato, soybean, rice and pine. LpTrx and PTrx were expressed as recombinant proteins in Escherichia coli and tested for thioredoxin activity. Both proteins displayed typical thioredoxin activity in the nonspecific insulin reduction assay, however, were not reduced by E. coli NADPK-dependant thioredoxin reductase.

引用

页码：7109 / 7117

页数：9

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