HIRIP3 is a nuclear phosphoprotein interacting with and phosphorylated by the serine-threonine kinase CK2

被引：13

作者：

Assrir, Nadine

Filhol, Odile

Galisson, Frederic

Lipinski, Marc ^{[1
]}

机构：

[1] Univ Paris 11, CNRS, UMR 8126, Inst Cancerol Gustave Roussy, F-94805 Villejuif, France

[2] CEA, Dept Reponse & Dynam Cellulaires, INSERM, F-38054 Grenoble, France

来源：

BIOLOGICAL CHEMISTRY | 2007年 / 388卷 / 04期

关键词：

HIRA; HIRA-interacting protein; nuclear protein; phosphoprotein; protein kinase CK2; protein-protein interaction;

D O I：

10.1515/BC.2007.045

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The HIRIP3 protein had been identified from its interaction with the HIRA histone chaperone. Experiments using anti-pepticle antisera indicated that this 556-aa protein is nuclear throughout the cell cycle and excluded from condensed chromatin during mitosis. Based on its electrophoretic migration and sensitivity to phosphatase treatment, endogenous HIRIP3 was found to be heavily phosphorylated. HIRIP3 can be phosphorylated in vitro by a recombinant form of the serine-threonine kinase CK2. Moreover, HIRIP3 protein was found to co-purify with a CK2 activity. Together, these data prompt us to propose HIRIP3 as a new member of the growing list of CK2 substrates with a possible role in chromatin metabolism.

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页码：391 / 398

页数：8

相关论文

共 38 条

[1]

Different roles of N-terminal and C-terminal halves of HIRA in transcription regulation of cell cycle-related genes that contribute to control of vertebrate cell growth [J].

Ahmad, A ;

Kikuchi, H ;

Takami, Y ;

Nakayama, T .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (37) :32090-32100

[2]

Genome-wide expression screens indicate a global role for protein kinase CK2 in chromatin remodeling [J].

Barz, T ;

Ackermann, K ;

Dubois, G ;

Eils, R ;

Pyerin, W .

JOURNAL OF CELL SCIENCE, 2003, 116 (08) :1563-1577

[3]

Large-scale characterization of HeLa cell nuclear phosphoproteins [J].

Beausoleil, SA ;

Jedrychowski, M ;

Schwartz, D ;

Elias, JE ;

Villén, J ;

Li, JX ;

Cohn, MA ;

Cantley, LC ;

Gygi, SP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (33) :12130-12135

[4]

Crystal structure of the human protein kinase CK2 regulatory subunit reveals its zinc finger-mediated dimerization [J].

Chantalat, L ;

Leroy, D ;

Filhol, O ;

Nueda, A ;

Benitez, MJ ;

Chambaz, EM ;

Cochet, C ;

Dideberg, O .

EMBO JOURNAL, 1999, 18 (11) :2930-2940

[5]

Structure and function of the conserved core of histone deposition protein Asf1 [J].

Daganzo, SM ;

Erzberger, JP ;

Lam, WM ;

Skordalakes, E ;

Zhang, RG ;

Franco, AA ;

Brill, SJ ;

Adams, PD ;

Berger, JM ;

Kaufman, PD .

CURRENT BIOLOGY, 2003, 13 (24) :2148-2158

[6]

Subnuclear localization and mitotic phosphorylation of HIRA, the human homologue of Saccharomyces cerevisiae transcriptional regulators Hir1p/Hir2p [J].

De Lucia, F ;

Lorain, S ;

Scamps, C ;

Galisson, F ;

Machold, J ;

Lipinski, M .

BIOCHEMICAL JOURNAL, 2001, 358 (02) :447-455

[7]

COEXPRESSION OF BOTH ALPHA-SUBUNIT AND BETA-SUBUNIT IS REQUIRED FOR ASSEMBLY OF REGULATED CASEIN KINASE-II [J].

FILHOL, O ;

COCHET, C ;

WEDEGAERTNER, P ;

GILL, GN ;

CHAMBAZ, EM .

BIOCHEMISTRY, 1991, 30 (46) :11133-11140

[8]

Functional genomic analysis of C-elegans chromosome I by systematic RNA interference [J].

Fraser, AG ;

Kamath, RS ;

Zipperlen, P ;

Martinez-Campos, M ;

Sohrmann, M ;

Ahringer, J .

NATURE, 2000, 408 (6810) :325-330

[9]

Glover CVC, 1998, PROG NUCLEIC ACID RE, V59, P95

[10]

Replication-independent histone deposition by the HIR complex and Asf1 [J].

Green, EM ;

Antczak, AJ ;

Bailey, AO ;

Franco, AA ;

Wu, KJ ;

Yates, JR ;

Kaufman, PD .

CURRENT BIOLOGY, 2005, 15 (22) :2044-2049

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