The action of diltiazem and gallopamil (D600) on calcium channel current and charge movement in mouse Purkinje neurons

The dihydropyridines (DHP) receptor forms a high threshold L-type calcium channel in various excitable cells. In skeletal and cardiac muscle cells, a DHP receptor antagonist blocks not only the voltage-gated calcium current but also immobilizes the charge movement linked to the receptor. The DHP receptor is also present in cerebellar Purkinje neurons. Previously, we showed that nifedipine immobilizes a part of the charge movement but has no effect on the calcium channel current recorded in freshly dissociated mice Purkinje neurons. We report here the effect of other families of DHP receptor antagonists, benzothiazepines and phenylalkylamines, on the physiological properties of this receptor in mouse Purkinje neurons. (C) 1998 Elsevier Science Ireland Ltd.