Peptide mimics elicit antibody responses against the outer-membrane lipooligosaccharide of group B Neisseria meningitidis

被引：29

作者：

Charalambous, BM

Feavers, IM

机构：

[1] Natl Inst Biol Stand & Controls, Div Bacteriol, Potters Bar EN6 3QG, Herts, England

[2] UCL, Royal Free & Univ Coll Med Sch, Dept Med Microbiol, London NW3 2PF, England

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 191卷 / 01期

关键词：

Neisseria meningitidis; serogroup B; lipooligosaccharide; vaccine; heptapeptide phage display; peptide mimic; resonant mirror biosensor;

D O I：

10.1016/S0378-1097(00)00368-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

As an alternative approach towards the development of a meningococcal vaccine, the potential of peptide mimics of lipooligosaccharide (LOS) to elicit cross-reactive immune responses against LOS was investigated. The heptapeptides SMYGSYN and APARQLP were identified by enrichment from a coliphage display library with a LOS-specific monoclonal antibody. Mice immunised with these peptides conjugated to diphtheria toroid elicited a total IgG response to LOS with geometric mean titres 2-4 times higher compared with nonimmunised controls. There was an increase in LOS-specific IgG1 immunoglobulin: whereas specific IgG2a and IgG3 decreased slightly in response to immunisation. The data demonstrated that peptide mimics can elicit immune responses against meningococcal LOS. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：45 / 50

页数：6

共 29 条

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