Enhanced cytokine generation by peripheral blood mononuclear cells in allergic and asthma subjects

被引:30
作者
Kenyon, NJ [1 ]
Kelly, EA [1 ]
Jarjour, NN [1 ]
机构
[1] Univ Wisconsin, Sch Med, Dept Med, Pulm & Crit Care Med Sect, Madison, WI 53792 USA
关键词
D O I
10.1016/S1081-1206(10)62450-7
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: Although preferential expression of the Th2 cytokines, IL-4 and IL-5, has been described in atopic asthma, the role of IFN-gamma and IL-10 are less clear. Objective: To determine the cytokine pattern of T cell mitogen-induced peripheral blood mononuclear cells obtained from atopic asthmatic (AA) subjects. Methods: Peripheral blood mononuclear cells obtained from AA (n = 24), allergic rhinitis (AR) (n = 9), and normals (NL) (n = 9) were stimulated with phytohemagglutinin (PHA) and the generation of IL-4, IL-5, IFN-gamma, IL-10, and GM-CSF was quantified using ELISA. Results: Compared with NL subjects, peripheral blood mononuclear cells from the atopic groups had increased generation of both IL-5 (AA, P = .001 and AR, P = .024) and IFN-gamma (AA, P = .037 and AR, P = .048) and decreased generation of IL-10 (AA, P = .038 and AR, P = .036). The absolute levels of cytokines did not differ between the two atopic groups; however, the ratio of IL-5/IL-10 was significantly higher in AA (P < .05), but not in AR when compared with NL subjects. Conclusion: The concomitant increase in the generation of IL-5 and IFN-gamma, with a decrease in IL-10 in the atopic groups suggests that in, at least a subset of these patients, there is potential expression of both Th2- and Th1-type cytokines. Furthermore, the increased IL-5 to IL-10 ratio could represent a key feature that distinguishes atopic asthmatic from non-asthmatic atopic subjects.
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收藏
页码:115 / 120
页数:6
相关论文
共 37 条
[1]   T(H)2 AND T(H)2-LIKE CELLS IN ALLERGY AND ASTHMA - PHARMACOLOGICAL PERSPECTIVES [J].
ANDERSON, GP ;
COYLE, AJ .
TRENDS IN PHARMACOLOGICAL SCIENCES, 1994, 15 (09) :324-332
[2]  
[Anonymous], 1987, AM REV RESPIR DIS, V136, P225
[3]   T-H1/T-H2 lymphocytes: Doubt some more [J].
Borish, L ;
Rosenwasser, L .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1997, 99 (02) :161-164
[4]   Interleukin-10 regulation in normal subjects and patients with asthma [J].
Borish, L ;
Aarons, A ;
Rumbyrt, J ;
Cvietusa, P ;
Negri, J ;
Wenzel, S .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1996, 97 (06) :1288-1296
[5]  
CALHOUN WJ, 1995, AM J RESP CRIT CARE, V151, P778
[6]   ELEVATED RELEASE OF TUMOR-NECROSIS-FACTOR-ALPHA AND INTERFERON-GAMMA BY BRONCHOALVEOLAR LEUKOCYTES FROM PATIENTS WITH BRONCHIAL-ASTHMA [J].
CEMBRZYNSKANOWAK, M ;
SZKLARZ, E ;
INGLOT, AD ;
TEODORCZYKINJEYAN, JA .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1993, 147 (02) :291-295
[7]   CD4 LYMPHOCYTE-T ACTIVATION IN ACUTE SEVERE ASTHMA - RELATIONSHIP TO DISEASE SEVERITY AND ATOPIC STATUS [J].
CORRIGAN, CJ ;
KAY, AB .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1990, 141 (04) :970-977
[8]   PERIPHERAL-BLOOD CD4 BUT NOT CD8 T-LYMPHOCYTES IN PATIENTS WITH EXACERBATION OF ASTHMA TRANSCRIBE AND TRANSLATE MESSENGER-RNA ENCODING CYTOKINES WHICH PROLONG EOSINOPHIL SURVIVAL IN THE CONTEXT OF A TH2-TYPE PATTERN - EFFECT OF GLUCOCORTICOID THERAPY [J].
CORRIGAN, CJ ;
HAMID, Q ;
NORTH, J ;
BARKANS, J ;
MOQBEL, R ;
DURHAM, S ;
GEMOUENGESAETH, V ;
KAY, AB .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1995, 12 (05) :567-578
[9]  
DELPRETE G, 1993, J IMMUNOL, V150, P353
[10]  
DURHAM SR, 1992, J IMMUNOL, V148, P2390