Dmrt1, a gene related to worm and fly sexual regulators, is required for mammalian testis differentiation

被引:562
作者
Raymond, CS
Murphy, MW
O'Sullivan, MG
Bardwell, VJ
Zarkower, D [1 ]
机构
[1] Univ Minnesota, Grad Program Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA
[3] Univ Minnesota, Dept Vet Med & Pathol, Minneapolis, MN 55455 USA
[4] Univ Minnesota, Ctr Canc, Minneapolis, MN 55455 USA
关键词
DM domain; Dmrt1; sex determination; doublesex; mab-3;
D O I
10.1101/gad.834100
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The only molecular similarity in sex determination found so far among phyla is between the Drosophila doublesex (dsx) and Caenorhabditis elegans mab-3 genes, dsx and mab-3 contain a zinc finger-like DNA-binding motif called the DM domain, perform several related regulatory functions, and are at least partially interchangeable in vivo. A DM domain gene called Dmrt1 has been implicated in male gonad development in a variety of vertebrates, on the basis of embryonic expression and chromosomal location. Such evidence is highly suggestive of a conserved role(s) for Dmrt1 in vertebrate sexual development, but there has been no functional analysis of this gene in any species. Here we show that murine Dmrt1 is essential for postnatal testis differentiation, with mutant phenotypes similar to those caused by human chromosome 9p deletions that remove the gene. As in the case of 9p deletions, Dmrt1 is dispensable for ovary development in the mouse. Thus, as in invertebrates, a DM domain gene regulates vertebrate male development.
引用
收藏
页码:2587 / 2595
页数:9
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