Protein splicing and autoproteolysis mechanisms

被引:136
作者
Perler, FB
Xu, MQ
Paulus, H
机构
[1] New England Biolabs Inc, Beverly, MA 01915 USA
[2] Boston Biomed Res Inst, Dept Muscle Res, Boston, MA 02114 USA
关键词
D O I
10.1016/S1367-5931(97)80065-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It has generally been assumed that the conversion of all inactive protein precursors to biologically active proteins is mediated by specific processing enzymes. However, numerous examples of self-catalyzed protein rearrangements have recently been discovered, including protein splicing and autoproteolysis of hedgehog proteins, glycosylasparaginases and pyruvoyl enzyme precursors. The initial formation of an ester bond by the acyl rearrangement of a peptide bond is a common feature of all of these autoprocessing reactions, which manifest themselves in diverse biological functions ranging from protein splicing to protein targeting, proenzyme activation, and the generation of enzyme-bound prosthetic groups. Although such acyl rearrangements are thermodynamically unfavorable, their coupling to diverse types of self-catalyzed irreversible steps drives the protein rearrangements to completion.
引用
收藏
页码:292 / 299
页数:8
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