A platform for brain-wide imaging and reconstruction of individual neurons

被引：286

作者：

Economon, Michael N. ^{[1
]}

Clack, Nathan G. ^{[1
]}

Levis, Luke D. ^{[1
]}

Gerfen, Charles R. ^{[1
,2
]}

Svoboda, Karel ^{[1
]}

Myers, Eugene W. ^{[1
,3
]}

Chandrashekar, Jayaram ^{[1
]}

机构：

[1] Howard Hughes Med Inst, Janelia Res Campus, Ashburn, VA USA

[2] NIMH, Lab Syst Neurosci, Bethesda, MD 20892 USA

[3] Max Planck Inst Mol Cell Biol & Genet, Dresden, Germany

来源：

ELIFE | 2016年 / 5卷

关键词：

OPTICAL SECTIONING TOMOGRAPHY; PRIMARY MOTOR CORTEX; MOUSE-BRAIN; IN-VIVO; FLUORESCENT PROTEINS; AXONAL PROJECTIONS; CORTICAL-NEURONS; THALAMIC NUCLEI; NERVOUS-SYSTEM; VIRAL VECTORS;

D O I：

10.7554/eLife.10566

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The structure of axonal arbors controls how signals from individual neurons are routed within the mammalian brain. However, the arbors of very few long-range projection neurons have been reconstructed in their entirety, as axons with diameters as small as 100 nm arborize in target regions dispersed over many millimeters of tissue. We introduce a platform for high-resolution, three-dimensional fluorescence imaging of complete tissue volumes that enables the visualization and reconstruction of long-range axonal arbors. This platform relies on a high-speed two-photon microscope integrated with a tissue vibratome and a suite of computational tools for large-scale image data. We demonstrate the power of this approach by reconstructing the axonal arbors of multiple neurons in the motor cortex across a single mouse brain.

引用

页数：22

共 70 条

[1]

Anderson JR, 2011, J MICROSC-OXFORD, V241, P13, DOI 10.1111/j.1365-2818.20.10.03402.x

[2] Network anatomy and in vivo physiology of visual cortical neurons [J].

Bock, Davi D. ;

Lee, Wei-Chung Allen ;

Kerlin, Aaron M. ;

Andermann, Mark L. ;

Hood, Greg ;

Wetzel, Arthur W. ;

Yurgenson, Sergey ;

Soucy, Edward R. ;

Kim, Hyon Suk ;

Reid, R. Clay .

NATURE, 2011, 471 (7337) :177-U59

[3] A Proposal for a Coordinated Effort for the Determination of Brainwide Neuroanatomical Connectivity in Model Organisms at a Mesoscopic Scale [J].

Bohland, Jason W. ;

Wu, Caizhi ;

Barbas, Helen ;

Bokil, Hemant ;

Bota, Mihail ;

Breiter, Hans C. ;

Cline, Hollis T. ;

Doyle, John C. ;

Freed, Peter J. ;

Greenspan, Ralph J. ;

Haber, Suzanne N. ;

Hawrylycz, Michael ;

Herrera, Daniel G. ;

Hilgetag, Claus C. ;

Huang, Z. Josh ;

Jones, Allan ;

Jones, Edward G. ;

Karten, Harvey J. ;

Kleinfeld, David ;

Kotter, Rolf ;

Lester, Henry A. ;

Lin, John M. ;

Mensh, Brett D. ;

Mikula, Shawn ;

Panksepp, Jaak ;

Price, Joseph L. ;

Safdieh, Joseph ;

Saper, Clifford B. ;

Schiff, Nicholas D. ;

Schmahmann, Jeremy D. ;

Stillman, Bruce W. ;

Svoboda, Karel ;

Swanson, Larry W. ;

Toga, Arthur W. ;

Van Essen, David C. ;

Watson, James D. ;

Mitra, Partha P. .

PLOS COMPUTATIONAL BIOLOGY, 2009, 5 (03)

[4]

Cai D, 2013, NAT METHODS, V10, P540, DOI [10.1038/NMETH.2450, 10.1038/nmeth.2450]

[5] TrakEM2 Software for Neural Circuit Reconstruction [J].

Cardona, Albert ;

Saalfeld, Stephan ;

Schindelin, Johannes ;

Arganda-Carreras, Ignacio ;

Preibisch, Stephan ;

Longair, Mark ;

Tomancak, Pavel ;

Hartenstein, Volker ;

Douglas, Rodney J. .

PLOS ONE, 2012, 7 (06)

[6]

Chung K, 2013, NAT METHODS, V10, P508, DOI [10.1038/NMETH.2481, 10.1038/nmeth.2481]

[7] SPONTANEOUS FIRING PATTERNS AND AXONAL PROJECTIONS OF SINGLE CORTICOSTRIATAL NEURONS IN THE RAT MEDIAL AGRANULAR CORTEX [J].

COWAN, RL ;

WILSON, CJ .

JOURNAL OF NEUROPHYSIOLOGY, 1994, 71 (01) :17-32

[8] Cell type-specific structural plasticity of axonal branches and boutons in the adult neocortex [J].

De Paola, V ;

Holtmaat, A ;

Knott, G ;

Song, S ;

Wilbrecht, L ;

Caroni, P ;

Svoboda, K .

NEURON, 2006, 49 (06) :861-875

[9] Ultramicroscopy:: three-dimensional visualization of neuronal networks in the whole mouse brain [J].

Dodt, Hans-Ulrich ;

Leischner, Ulrich ;

Schierloh, Anja ;

Jaehrling, Nina ;

Mauch, Christoph Peter ;

Deininger, Katrin ;

Deussing, Jan Michael ;

Eder, Matthias ;

Zieglgaensberger, Walter ;

Becker, Klaus .

NATURE METHODS, 2007, 4 (04) :331-336

[10] QUANTITATIVE STUDIES OF THE AUTOFLUORESCENCE DERIVED FROM NEURONAL LIPOFUSCIN [J].

DOWSON, JH ;

HARRIS, SJ .

JOURNAL OF MICROSCOPY-OXFORD, 1981, 123 (SEP) :249-258

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