Free fetal DNA in maternal plasma in anembryonic pregnancies: confirmation that the origin is the trophoblast

被引:279
作者
Alberry, M.
Maddocks, D.
Jones, M.
Hadi, M. Abdel
Abdel-Fattah, S.
Avent, N.
Soothill, P. W.
机构
[1] St Michaels Hosp, Dept Obstet & Gynaecol, Bristol BS2 8EG, Avon, England
[2] Ain Shams Univ, Matern Hosp, Cairo, Egypt
[3] Univ W England, Fac Sci Appl, Biomed Res Ctr, Bristol BS16 1QY, Avon, England
[4] Southmead Gen Hosp, Bristol Genet Lab, Bristol, Avon, England
关键词
free fetal DNA; anembryonic gestation; PCR;
D O I
10.1002/pd.1700
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Objective To test the hypothesis that free fetal DNA (ffDNA) circulating in maternal plasma originates mainly from the placenta we studied ffDNA levels in anembryonic pregnancies. Methods Maternal blood samples were collected from 15 normal first-trimester pregnancies in which fetal sex was subsequently determined and nine patients with a diagnosis of anembryonic gestation (AG). The Y chromosome DYS14 gene was quantified by real-time quantitative PCR (RT-PCR) for the determination of fetal sex in both plasma and chorionic tissue samples. Fetal sex in chorionic tissue samples was also determined using quantitative fluorescence PCR (QF-PCR). Results The correct sex result was obtained from maternal plasma in all. Four AG pregnancies were female (DYS14 negative) results. In five of the AG cases, the chorionic tissue was found to be male (by both QF-PCR and RT-PCR which agreed) and positive male signal was found in maternal plasma by RT-PCR. There was no statistical difference between median free fetal DNA concentration in plasma between the AG male cases (148.3 GE/mL) and controls (145.8 GE/mL). Conclusion Since ffDNA levels are normal in pregnancies without a fetus, the data support the hypothesis that the trophoblastic cells are the major source ffDNA in maternal plasma. Copyright (c) 2007 John Wiley & Sons, Ltd.
引用
收藏
页码:415 / 418
页数:4
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