Genotoxicity evaluation of heat shock in gold fish (Carassius auratus)

被引：38

作者：

Anitha, B ^{[1
]}

Chandra, N

Gopinath, PM

Durairaj, G

机构：

[1] Cent Leather Res Inst, Dept Biochem, Adyar 600113, Chennai, India

[2] ALPGIBMS, Dept Genet, Chennai 600113, India

[3] Univ Madras, Dept Zool, Madras, Tamil Nadu, India

来源：

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 2000年 / 469卷 / 01期

关键词：

heat shock; Carassius auratus; nuclear anomalies; comet assay chromosomal aberrations;

D O I：

10.1016/S1383-5718(00)00029-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genotoxicity evaluation of heat shock was carried out in Carassius auratus. The genotoxicity end points studied were nuclear anomalies (micronucleus assay), chromosomal aberrations, DNA damage (comet assay) and cell proliferation. The heat shock temperatures used were 34 degrees C, 36 degrees C and 38 degrees C. The results demonstrated that heat shock causes the induction of micronucleus at all the three temperature studied. Heat shock also inhibited cell proliferation at 38 degrees C acid caused aberrations in the metaphase chromosomes at 34 degrees C and 36 degrees C. Comet assay demonstrated single strand DNA damage at all the three temperatures. The results obtained indicate that heat shock is a genotoxicant. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：1 / 8

页数：8

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