ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan

被引：230

作者：

Kuno, K

Okada, Y

Kawashima, H

Nakamura, H

Miyasaka, M

Ohno, H

Matsushima, K

机构：

[1] Kanazawa Univ, Canc Res Inst, Dept Mol Membrane Biol, Kanazawa, Ishikawa 9200934, Japan

[2] Keio Univ, Sch Med, Dept Pathol, Shinjuku Ku, Tokyo 1600016, Japan

[3] Osaka Univ, Grad Sch Med, Dept Bioregulat, Biomed Res Ctr, Suita, Osaka 5650871, Japan

[4] Univ Tokyo, Sch Med, Dept Mol Prevent Med, Bunkyo Ku, Tokyo 113, Japan

来源：

FEBS LETTERS | 2000年 / 478卷 / 03期

关键词：

aggrecan; disintegrin and metalloproteinase thrombospondin; extracellular matrix; cartilage degradation;

D O I：

10.1016/S0014-5793(00)01854-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A disintegrin-like and metalloproteinase with thrombospondin type I motifs-1 (ADAMTS-1) is an extracellular matrix-anchored metalloproteinase. In this study we have demonstrated that ADAMTS-1 is able to cleave a major cartilage proteoglycan, aggrecan, N-terminal sequencing analysis of the cleavage product revealed that ADAMTS-1 cleaves the Glu(1871)-Leu(1872) bond within the chondroitin sulfate attachment domain of aggrecan, In addition, deletional analysis demonstrated that the C-terminal spacer region of ADAMTS-1 is necessary to degrade aggrecan, These results suggest that ADAMTS-1 may be involved in the turnover of aggrecan in vivo. (C) 2000 Federation of European Biochemical Societies, Published by Elsevier Science B.V. All rights reserved.

引用

页码：241 / 245

页数：5

共 30 条

[1] Cloning and characterization of ADAMTS11, an aggrecanase from the ADAMTS family [J].