The kinase MSK1 is required for induction of c-fos by lysophosphatidic acid in mouse embryonic stem cells -: art. no. 6

被引：50

作者：

Schuck, S

Soloaga, A

Schratt, G

Arthur, JSC

Nordheim, A

机构：

[1] Univ Tubingen, Mol Biol Abt, Interfak Inst Zellbiol, D-72076 Tubingen, Germany

[2] Univ Dundee, Dept Biochem, MRC, Prot Phosphorylat Unit, Dundee DD1 5EH, Scotland

来源：

BMC MOLECULAR BIOLOGY | 2003年 / 4卷

关键词：

D O I：

10.1186/1471-2199-4-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: The regulation of the immediate-early gene c-fos serves as a paradigm for signal-activated gene induction. Lysophosphatidic acid is a potent serum-borne mitogen able to induce c-fos. Results: Analysing the signalling events following stimulation of mouse embryonic stem cells with serum and lysophosphatidic acid, we show that the extracellular signal-regulated kinase (ERK) pathway is involved in mediating c-fos induction. We demonstrate that the ERK-activated kinase MSK1 is required for full c-fos promoter activation, as well as for the phosphorylation of cAMP-responsive element (CRE) binding proteins. We propose that MSK1 contributes to ERK-mediated c-fos promoter activation by targeting CRE binding proteins. Conclusion: These results show that MSK1 is an important ERK-activated mediator of mitogen-stimulated c-fos induction. In addition, they indicate that MSK1 could act through CRE binding proteins to achieve c-fos promoter activation. Thus, they further our understanding of the complex regulation of the model immediate-early gene c-fos.

引用

收藏

页数：8

相关论文

共 33 条

[1] A dominant-negative inhibitor of CREB reveals that it is a general mediator of stimulus-dependent transcription of c-fos [J].

Ahn, S ;

Olive, M ;

Aggarwal, S ;

Krylov, D ;

Ginty, DD ;

Vinson, C .

MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (02) :967-977

[2] Activation of SRF-regulated chromosomal templates by Rho-family GTPases requires a signal that also induces H4 hyperacetylation [J].

Alberts, AS ;

Geneste, O ;

Treisman, R .

CELL, 1998, 92 (04) :475-487

[3] The protein kinase C inhibitors Ro 318220 and GF 109203X are equally potent inhibitors of MAPKAP kinase-1 beta (Rsk-2) and p70 S6 kinase [J].

Alessi, DR .

FEBS LETTERS, 1997, 402 (2-3) :121-123

[4] PD-098059 IS A SPECIFIC INHIBITOR OF THE ACTIVATION OF MITOGEN-ACTIVATED PROTEIN-KINASE KINASE IN-VITRO AND IN-VIVO [J].

ALESSI, DR ;

CUENDA, A ;

COHEN, P ;

DUDLEY, DT ;

SALTIEL, AR .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (46) :27489-27494

[5] MSK1 is required for CREB phosphorylation in response to mitogens in mouse embryonic stem cells [J].

Arthur, JSC ;

Cohen, P .

FEBS LETTERS, 2000, 482 (1-2) :44-48

[6] SERINE 133-PHOSPHORYLATED CREB INDUCES TRANSCRIPTION VIA A COOPERATIVE MECHANISM THAT MAY CONFER SPECIFICITY TO NEUROTROPHIN SIGNALS [J].

BONNI, A ;

GINTY, DD ;

DUDEK, H ;

GREENBERG, ME .

MOLECULAR AND CELLULAR NEUROSCIENCE, 1995, 6 (02) :168-183

[7] Cdc42 is required for PIP2-induced actin polymerization and early development but not for cell viability [J].

Chen, F ;

Ma, L ;

Parrini, MC ;

Mao, X ;

Lopez, M ;

Wu, C ;

Marks, PW ;

Davidson, L ;

Kwiatkowski, DJ ;

Kirchhausen, T ;

Orkin, SH ;

Rosen, FS ;

Mayer, BJ ;

Kirschner, MW ;

Alt, FW .

CURRENT BIOLOGY, 2000, 10 (13) :758-765

[8] Phosphoacetylation of histone H3 on c-fos- and c-jun-associated nucleosomes upon gene activation [J].

Clayton, AL ;

Rose, S ;

Barratt, MJ ;

Mahadevan, LC .

EMBO JOURNAL, 2000, 19 (14) :3714-3726

[9] Specificity and mechanism of action of some commonly used protein kinase inhibitors [J].

Davies, SP ;

Reddy, H ;

Caivano, M ;

Cohen, P .

BIOCHEMICAL JOURNAL, 2000, 351 (351) :95-105

[10] Rsk-2 activity is necessary for epidermal growth factor-induced phosphorylation of CREB protein and transcription of c-fos gene [J].

De Cesare, D ;

Jacquot, S ;

Hanauer, A ;

Sassone-Corsi, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (21) :12202-12207

← 1 2 3 4 →