DONGLE and DEFECTIVE IN ANTHER DEHISCENCE1 Lipases Are Not Essential for Wound- and Pathogen-Induced Jasmonate Biosynthesis: Redundant Lipases Contribute to Jasmonate Formation

被引:91
作者
Ellinger, Dorothea [1 ]
Stingl, Nadja [2 ]
Kubigsteltig, Ines Ingeborg [1 ]
Bals, Thomas [1 ]
Juenger, Melanie [1 ]
Pollmann, Stephan [1 ]
Berger, Susanne [2 ]
Schuenemann, Danja [1 ]
Mueller, Martin Johannes [2 ]
机构
[1] Ruhr Univ Bochum, Dept Plant Physiol, D-44801 Bochum, Germany
[2] Univ Wurzburg, Biozentrum, Dept Pharmaceut Biol, D-97082 Wurzburg, Germany
关键词
OXO-PHYTODIENOIC ACID; ARABIDOPSIS MUTANT CEV1; ALLENE OXIDE SYNTHASE; SUBCELLULAR-LOCALIZATION; HYPERSENSITIVE RESPONSE; SIGNAL-TRANSDUCTION; THALIANA; ACCUMULATION; GALACTOLIPIDS; BIOGENESIS;
D O I
10.1104/pp.110.155093
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Lipases are involved in the generation of jasmonates, which regulate responses to biotic and abiotic stresses. Two sn-1-specific acyl hydrolases, DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1) and DONGLE (DGL), have been reported to be localized in plastids and to be essential and sufficient for jasmonate biosynthesis in Arabidopsis (Arabidopsis thaliana) leaves. Here, we show that levels of 12-oxo-phytodienoic acid (OPDA) and jasmonic acid in three different DGL RNA interference lines and the dad1 mutant were similar to wild-type levels during the early wound response as well as after Pseudomonas infection. Due to the lack of sn-2 substrate specificity, synthesis of dinor OPDA was not expected and also not found to be affected in DGL knockdown and DGL-overexpressing lines. As reported, DAD1 participates in jasmonate formation only in the late wound response. In addition, DGL protein was found to be localized in lipid bodies and not in plastids. Furthermore, jasmonate levels in 16 additional mutants defective in the expression of lipases with predicted chloroplast localization did not show strong differences from wild-type levels after wounding, except for a phospholipase A (PLA) PLA-I gamma 1 (At1g06800) mutant line that displayed diminished wound-induced dinor OPDA, OPDA, and jasmonic acid levels. A quadruple mutant defective in four DAD1-like lipases displayed similar jasmonate levels as the mutant line of PLA-I gamma 1 after wounding. Hence, we identify PLA-I gamma 1 as a novel target gene to manipulate jasmonate biosynthesis. Our results suggest that, in addition to DAD1 and PLA-I gamma 1, still unidentified enzymes with sn-1 and sn-2 hydrolase activity are involved in wound-and pathogen-induced jasmonate formation, indicating functional redundancy within the lipase family.
引用
收藏
页码:114 / 127
页数:14
相关论文
共 43 条
[1]   Oxylipin profiling of the hypersensitive response in Arabidopsis thaliana -: Formation of a novel oxo-phytodienoic acid-containing galactolipid, arabidopside E [J].
Andersson, Mats X. ;
Hamberg, Mats ;
Kourtchenko, Olga ;
Brunnstrom, Asa ;
McPhail, Kerry L. ;
Gerwick, William H. ;
Goebel, Cornelia ;
Feussner, Ivo ;
Ellerstrom, Mats .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (42) :31528-31537
[2]  
[Anonymous], 2002, ARABIDOPSIS BOOK
[3]  
[Anonymous], 1989, Molecular Cloning: A Laboratory
[4]  
Ausubel FM, 1995, CURRENT PROTOCOLS MO
[5]  
Bechtold N, 1998, METH MOL B, V82, P259
[6]   A CHLOROPLAST LIPOXYGENASE IS REQUIRED FOR WOUND-INDUCED JASMONIC ACID ACCUMULATION IN ARABIDOPSIS [J].
BELL, E ;
CREELMAN, RA ;
MULLET, JE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (19) :8675-8679
[7]   cyclo-Oxylipin-galactolipids in plants:: occurrence and dynamics [J].
Boettcher, Christine ;
Weiler, Elmar W. .
PLANTA, 2007, 226 (03) :629-637
[8]   Plant oxylipins: Plant responses to 12-oxo-phytodienoic acid are governed by its specific structural and functional properties [J].
Boettcher, Christine ;
Pollmann, Stephan .
FEBS JOURNAL, 2009, 276 (17) :4693-4704
[9]   Wounding stimulates the accumulation of glycerolipids containing oxophytodienoic acid and dinor-oxophytodienoic acid in Arabidopsis leaves [J].
Buseman, Christen M. ;
Tamura, Pamela ;
Sparks, Alexis A. ;
Baughman, Ethan J. ;
Maatta, Sara ;
Zhao, Jian ;
Roth, Mary R. ;
Esch, Steven Wynn ;
Shah, Jyoti ;
Williams, Todd D. ;
Welti, Ruth .
PLANT PHYSIOLOGY, 2006, 142 (01) :28-39
[10]   Subcellular localisation of Medicago truncatula 9/13-hydroperoxide lyase reveals a new localisation pattern and activation mechanism for CYP74C enzymes [J].
De Domenico, Stefania ;
Tsesmetzis, Nicolas ;
Di Sansebastiano, Gian Pietro ;
Hughes, Richard K. ;
Casey, Rod ;
Santino, Angelo .
BMC PLANT BIOLOGY, 2007, 7 (1)