Impedance and shear wave resonance analysis of ligand-receptor interactions at functionalized surfaces and of cell monolayers

被引：44

作者：

Steinem, C ^{[1
]}

Janshoff, A ^{[1
]}

Wegener, J ^{[1
]}

Ulrich, WP ^{[1
]}

Willenbrink, W ^{[1
]}

Sieber, M ^{[1
]}

Galla, HJ ^{[1
]}

机构：

[1] Univ Munster, Inst Biochem, D-48149 Munster, Germany

来源：

BIOSENSORS & BIOELECTRONICS | 1997年 / 12卷 / 08期

关键词：

impedance spectroscopy; quartz-crystal microbalance; solid supported membranes; ganglioside-lectin interaction; ganglioside-toxin interaction; cell adhesion; epithelial barrier function;

D O I：

10.1016/S0956-5663(97)00045-6

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The present paper scrutinizes the application of impedance spectroscopy and quartz-crystal microbalance (QCM) measurements in the analysis of composite layers of receptor containing lipid bilayers, and their interaction with external ligands or pore-forming peptides. The formation of supramolecular structures and their analysis will be discussed. Impedance measurement allows one to follow the adsorption of proteins on artificial membranes. This method is even more suitable for quantifying changes in membrane conductivity induced by channel peptides incorporated into the lipid membrane. The QCM is another sophisticated method for analyzing ganglioside-lectin and ganglioside-toxin interactions. A critical comparison between both methods will be given. Moreover, we will demonstrate that the QCM method, especially in combination with impedance analysis, is a completely new approach for determining electrical and viscoelastic properties of epithelial and endothelial cell monolayers that form controlled barriers in vivo. (C) 1997 Elsevier Science Limited.

引用

页码：787 / 808

页数：22

共 65 条

[1] FORMATION OF MONOLAYER FILMS BY THE SPONTANEOUS ASSEMBLY OF ORGANIC THIOLS FROM SOLUTION ONTO GOLD [J].