Quantitative super-resolution microscopy: pitfalls and strategies for image analysis

被引：55

作者：

Durisic, Nela ^{[1
]}

Cuervo, Lara Laparra ^{[1
]}

Lakadamyali, Melike ^{[1
]}

机构：

[1] ICFO Inst Ciencies Foton, Castelldefels 08860, Barcelona, Spain

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2014年 / 20卷

关键词：

PHOTOACTIVATED LOCALIZATION MICROSCOPY; PAIR CORRELATION-ANALYSIS; SPATIAL-ORGANIZATION; FLUORESCENT-PROBES; PROTEIN; RESOLUTION; CELLS; PALM; GFP; STOICHIOMETRY;

D O I：

10.1016/j.cbpa.2014.04.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Super-resolution microscopy is an enabling technology that allows biologists to visualize cellular structures at nanometer length scales using far-field optics. To break the diffraction barrier, it is necessary to leverage the distinct molecular states of fluorescent probes. At the same time, the existence of these different molecular states and the photophysical properties of the fluorescent probes can complicate data quantification and interpretation. Here, we review the pitfalls in super-resolution data analysis that must be avoided for proper interpretation of images.

引用

页码：22 / 28

页数：7

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