Motility of ram spermatozoa during storage in a chemically-defined diluent containing antioxidants

被引:53
作者
Upreti, GC [1 ]
Jensen, K [1 ]
Oliver, JE [1 ]
Duganzich, DM [1 ]
Munday, R [1 ]
Smith, JF [1 ]
机构
[1] AgResearch, Dairy & Beef Div, Ruakura Agr Res Ctr, Hamilton 3123, New Zealand
关键词
sheep male reproduction; antioxidants; spermatozoa; motility; pyruvate; vitamin E; BHA; desferal; n-propyl gallate; catalase;
D O I
10.1016/S0378-4320(97)00054-7
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The effects of five antioxidants-Vitamin E (VE), butylated hydroxyanisole (BHA), n-propyl gallate (n-PG), deferoxamine mesylate (Desferal) and catalase (EC 1.11.1.6)-on the maintenance of motility of ram spermatozoa in a chemically-defined ram semen diluent (RSD-1) have been evaluated. VE, n-PG and Desferal inhibited spermatozoal motility. The relative inhibition (i.e., ratio of change in % motility over 24 h between the treatment group and the corresponding control) at equimolar concentrations (100 mu M) of Desferal, VE and n-PG were 1.6, 1.8 and 3.6 respectively. BHA had no effect at 10 mu M but at lower concentrations, gave a slight improvement in motility in freshly diluted spermatozoal samples and in those stored for 1 day at 15 degrees C. The addition of catalase to RSD-1 was also ineffective in improving the motility of spermatozoa. The lack of beneficial effects of the tested antioxidants suggests that RSD-1 itself may destroy reactive oxygen species and the antioxidant activity of RSD-1 components requires further study. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:269 / 278
页数:10
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