Antioxidant measurements

被引:170
作者
Somogyi, Aniko [1 ]
Rosta, Klara
Pusztai, Peter
Tulassay, Zsolt
Nagy, Geza
机构
[1] Semmelweis Univ, Dept Internal Med 2, Budapest, Hungary
[2] Semmelweis Univ, Dept Med Chem Mol Biol & Pathobiol, H-1085 Budapest, Hungary
[3] Semmelweis Univ, Hungarian Acad Sci, Clin Gastroenterol Res Unit, Budapest, Hungary
关键词
oxidative stress; antioxidant; measurement; antioxidant capacity;
D O I
10.1088/0967-3334/28/4/R01
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Chemical reactions, including oxidation and reduction of molecules, occur in every cell. These reactions can lead to the production of free radicals. Free radicals react with organic substrates such as lipids, proteins, and DNA. Through oxidation free radicals cause damage to these molecules, disturbing their normal function, and may therefore contribute to a variety of diseases. The anti-oxidation system, which consists of enzymatic antioxidants and nonenzymatic antioxidants, defends against oxidative stress. The aim of this review is to summarize general aspects of methods to measure the antioxidant defence system all in one (total antioxidant capacity) and discuss a number of methods which are currently used for detection of antioxidant properties.
引用
收藏
页码:R41 / R55
页数:15
相关论文
共 69 条
[1]  
Alho H, 1998, RESTOR NEUROL NEUROS, V12, P159
[2]   ENDOGENOUS DNA DAMAGE AS RELATED TO CANCER AND AGING [J].
AMES, BN .
MUTATION RESEARCH, 1989, 214 (01) :41-46
[3]   DNA damage responses to oxidative stress [J].
Barzilai, A ;
Yamamoto, KI .
DNA REPAIR, 2004, 3 (8-9) :1109-1115
[4]  
BECKMAN JS, 1994, METHOD ENZYMOL, V233, P229
[5]  
Benzie IFF, 1999, METHOD ENZYMOL, V299, P15
[6]   The ferric reducing ability of plasma (FRAP) as a measure of ''antioxidant power'': The FRAP assay [J].
Benzie, IFF ;
Strain, JJ .
ANALYTICAL BIOCHEMISTRY, 1996, 239 (01) :70-76
[7]   Antioxidants, oxidative damage and oxygen deprivation stress: a review [J].
Blokhina, O ;
Virolainen, E ;
Fagerstedt, KV .
ANNALS OF BOTANY, 2003, 91 (02) :179-194
[8]   BIOLOGICAL ANTIOXIDANTS [J].
BURTON, GW ;
FOSTER, DO ;
PERLY, B ;
SLATER, TF ;
SMITH, ICP ;
INGOLD, KU .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1985, 311 (1152) :565-578
[9]   A modified amperometric electrode for the determination of free radicals [J].
Campanella, L ;
Favero, G ;
Tomassetti, M .
SENSORS AND ACTUATORS B-CHEMICAL, 1997, 44 (1-3) :559-565
[10]  
CAO G, 1995, CLIN CHEM, V41, P1738