Comparison of gene expression of umbilical cord vein and bone marrow-derived mesenchymal stem cells

被引:225
作者
Panepucci, RA
Siufi, JLC
Silva, WA
Proto-Siquiera, R
Neder, L
Orellana, M
Rocha, V
Covas, DT
Zago, MA
机构
[1] Ctr Cell Therapy & Reg Blood Ctr, Dept Clin Med, Ribeirao Preto, Brazil
[2] Fac Med, Dept Pathol, Ribeirao Preto, Brazil
[3] Hop St Louis, Bone Marrow Transplant Unit, Paris, France
关键词
mesenchymal stem cells; gene expression; umbilical cord; angiogenesis;
D O I
10.1634/stemcells.2004-0024
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Mesenchymal stem cells (MSCs) give origin to the marrow stromal environment that supports hematopoiesis. These cells present a wide range of differentiation potentials and a complex relationship with hematopoietic stem cells (HSCs) and endothelial cells. In addition to bone marrow (BM), MSCs can be obtained from other sites in the adult or the fetus. We isolate MSCs from the umbilical cord (UC) veins that are morphologically and immunophenotpically similar to MSCs obtained from the BM. In culture,. these cells are capable of differentiating in vitro into adipocytes, osteoblasts, and condrocytes. The gene expression profiles of BM-MSCs and of UC-MSCs were compared by serial analysis of gene expression, then validated by reverse transcription polymerase chain reaction of selected genes. The two lineages shared almost all of the first thousand most expressed transcripts, including vimentin, galectin 1, osteonectin, collagens, transgelins, annexin A2, and MMP2. Nevertheless, a set of genes related to antimicrobial activity and to osteogenesis was more expressed in BM-MSCs, whereas higher expression in UC-MSCs was observed for genes that participate in pathways related to matrix remodeling via metalloproteinases and angiogenesis. Finally, cultured endothelial cells, CD34(+)HSCs, MSCs, blood leukocytes, and bulk BM clustered together, separated from seven other normal nonhematopoietic tissues, on the basis of shared expressed genes. MSCs isolated from UC veins are functionally similar to BM-MSCs, but differentially expressed genes may reflect differences related to their sites of origin: BM-MSCs would be more committed to osteogenesis, whereas UC-MSCs would be more committed to angiogenesis.
引用
收藏
页码:1263 / 1278
页数:16
相关论文
共 83 条
[1]  
Anker PSI, 2003, HAEMATOLOGICA, V88, P845
[2]   Transplanted adult hematopoietic stems cells differentiate into functional endothelial cells [J].
Bailey, AS ;
Jiang, SG ;
Afentoulis, M ;
Baumann, CI ;
Schroeder, DA ;
Olson, SB ;
Wong, MH ;
Fleming, WH .
BLOOD, 2004, 103 (01) :13-19
[3]   Zyxin: zinc fingers at sites of cell adhesion [J].
Beckerle, MC .
BIOESSAYS, 1997, 19 (11) :949-957
[4]  
Brekken Rolf A., 2001, Matrix Biology, V19, P816
[5]   Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow [J].
Campagnoli, C ;
Roberts, IAG ;
Kumar, S ;
Bennett, PR ;
Bellantuono, I ;
Fisk, NM .
BLOOD, 2001, 98 (08) :2396-2402
[6]   Bone morphogenetic proteins, their antagonists, and the skeleton [J].
Canalis, E ;
Economides, AN ;
Gazzerro, E .
ENDOCRINE REVIEWS, 2003, 24 (02) :218-235
[7]   Actin-based motility as a self-organized system: mechanism and reconstitution in vitro [J].
Carlier, MF ;
Wiesner, S ;
Le Clainche, C ;
Pantaloni, D .
COMPTES RENDUS BIOLOGIES, 2003, 326 (02) :161-170
[8]   Selective adhesion of osteoblastic cells to different integrin ligands induces osteopontin gene expression [J].
Carvalho, RS ;
Kostenuik, PJ ;
Salih, E ;
Bumann, A ;
Gerstenfeld, LC .
MATRIX BIOLOGY, 2003, 22 (03) :241-249
[9]   Characterization of the molecular interactions of interleukin-8 (CXCL8), growth related oncogen α (CXCL1) and a non-peptide antagonist (SB 225002) with the human CXCR2 [J].
Catusse, J ;
Liotard, A ;
Loillier, B ;
Pruneau, D ;
Paquet, JL .
BIOCHEMICAL PHARMACOLOGY, 2003, 65 (05) :813-821
[10]   Biglycan-deficient mice have delayed osteogenesis after marrow ablation [J].
Chen, XD ;
Allen, MR ;
Bloomfield, S ;
Xu, T ;
Young, M .
CALCIFIED TISSUE INTERNATIONAL, 2003, 72 (05) :577-582