Hydrocortisone reinforces the blood-brain barrier properties in a serum free cell culture system

被引：182

作者：

Hoheisel, D ^{[1
]}

Nitz, T ^{[1
]}

Franke, H ^{[1
]}

Wegener, J ^{[1
]}

Hakvoort, A ^{[1
]}

Tilling, T ^{[1
]}

Galla, HJ ^{[1
]}

机构：

[1] Univ Munster, Inst Biochem, D-4400 Munster, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 244卷 / 01期

关键词：

D O I：

10.1006/bbrc.1997.8051

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The increasing number of newly developed drugs demands for functional in vitro models of the blood-brain barrier to determine their brain uptake. Cultured cerebral capillary endothelial cells are considered to be such a model, however in serum containing media they exhibit low electrical resistances and high permeabilities compared to the in vivo situation. Here we report the establishment of a serum-free cell culture model. Withdrawal of serum already caused a twofold increase of transendothelial resistance (TER), which in presence of serum is about 100-150 Ohm . cm(2). We tested several supplements and found that hydrocortisone is a potent stimulator for the formation of barrier properties. TERs up to 1000 Ohm . cm(2) were measured in the presence of physiological relevant hydrocortisone concentrations. In correspondence to the TER increase hydrocortisone decreased cell monolayer permeability for sucrose down to 5 . 10(-7) cm/s, which is close to the in vivo value of 1.2 . 10(-7) cm/s and by a factor of five lower compared to cultures without hydrocortisone and in presence of serum. (C) 1998 Academic Press.

引用

页码：312 / 316

页数：5

共 12 条

[1] BRAIN MICROVESSEL ENDOTHELIAL-CELLS IN TISSUE-CULTURE - A MODEL FOR STUDY OF BLOOD-BRAIN-BARRIER PERMEABILITY [J].