Copy number related transgene expression and mosaic somatic expression in hemizygous and homozygous transgenic tilapia (Oreochromis niloticus)

被引:43
作者
Rahman, MA [1 ]
Hwang, GL [1 ]
Razak, SA [1 ]
Sohm, F [1 ]
Maclean, N [1 ]
机构
[1] Univ Southampton, Sch Biol Sci, Div Cell Sci, Southampton SO16 7PX, Hants, England
关键词
beta-galactosidase; mosaic expression; transgenic fish;
D O I
10.1023/A:1026517212807
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Three lines of transgenic tilapia (Oreochromisniloticus) fish were generated with a constructcontaining a lacZ reporter gene spliced to a 4.7 kb 5' regulatory region of a carp beta actin gene. All these three lines contain different copy numbers of transgenes and the levels of lacZ expression were found to be related to transgene copy number. Mosaic patterns of somatic lacZ expression were observed in these three lines which differed between lines but were consistent within a line. We also observed that expression of the reporter gene in homozygous transgenic fish was approximately two-fold greater than in the hemizygous transgenics. Analysis of expression of the reporter gene on a tissue-to-tissue basis demonstrated that lacZ expression of the reporter gene in stably transformed fish occured with variable intensity in different organs and tissues and was also sometimes variable in different cells of the same tissue in G1 and G2 generations of the transgenic lines.
引用
收藏
页码:417 / 427
页数:11
相关论文
共 27 条
[1]   SHORT-TERM CELL-CULTURE TECHNIQUE FOR OBTAINING CHROMOSOMES IN MARINE AND FRESH-WATER FISH [J].
ALVAREZ, MC ;
OTIS, J ;
AMORES, A ;
GUISE, K .
JOURNAL OF FISH BIOLOGY, 1991, 39 (06) :817-824
[2]   EPIGENETIC REPEAT-INDUCED GENE SILENCING (RIGS) IN ARABIDOPSIS [J].
ASSAAD, FF ;
TUCKER, KL ;
SIGNER, ER .
PLANT MOLECULAR BIOLOGY, 1993, 22 (06) :1067-1085
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]  
BRAELL WA, 1991, TECHNICAL B, V129
[5]   The yeast HPR1 gene has a functional role in transcriptional elongation that uncovers a novel source of genome instability [J].
Chávez, S ;
Aguilera, A .
GENES & DEVELOPMENT, 1997, 11 (24) :3459-3470
[6]   lacZ sequences prevent regulated expression of housekeeping genes [J].
Cohen-Tannoudji, M ;
Vandormael-Pournin, S ;
Drezen, JM ;
Mercier, P ;
Babinet, C ;
Morello, D .
MECHANISMS OF DEVELOPMENT, 2000, 90 (01) :29-39
[7]   Modulation of plasmid DNA methylation and expression in zebrafish embryos [J].
Collas, P .
NUCLEIC ACIDS RESEARCH, 1998, 26 (19) :4454-4461
[8]  
DECARVALHO F, 1992, EMBO J, V11, P2995
[9]   Variegated gene expression in mice [J].
Dobie, K ;
Mehtali, M ;
McClenaghan, M ;
Lathe, R .
TRENDS IN GENETICS, 1997, 13 (04) :127-130
[10]   Do transgene arrays form heterochromatin in vertebrates? [J].
Dorer, DR .
TRANSGENIC RESEARCH, 1997, 6 (01) :3-10