Ion chromatography of phytate in roots and tubers

被引：28

作者：

Phillippy, BQ

Bland, JM

Evens, TJ

机构：

[1] USDA ARS, So Reg Res Ctr, Commod Utilizat Res Grp, New Orleans, LA 70124 USA

[2] USDA ARS, So Reg Res Ctr, Formosan Subterranean Termite Res Grp, New Orleans, LA 70124 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2003年 / 51卷 / 02期

关键词：

phytic acid; phytate; inositol hexakisphosphate; potato; Solanum tuberosum;

D O I：

10.1021/jf025827m

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The ion chromatographic method for the quantification of phytate (InsP(6)) in foods was adapted for the analysis of roots and tubers. To maximize sensitivity, ultraviolet (UV) detection following postcolumn derivatization was compared with evaporative light-scattering detection (ELSD). Detection limits for phytate were 0.5 and 1 mug for UV and ELSD, respectively. Unidentified peaks eluting close to and after InSP6 were removed by solid-phase extraction. Phytate was detected in 11 of 15 roots or tubers. The highest phytate levels were 0.169 and 0.133% of the fresh weight of taro (Colocasia esculenta) and yuca (Manihot esculenta), respectively. Potatoes (Solanum tuberosum) contained 0.035-0.073% phytate, whereas no phytate at a detection limit of 0.003% of fresh weight was observed in sweet potatoes (Ipomoea batatas).

引用

页码：350 / 353

页数：4

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