Identification of neuropeptide FF-related peptides in human cerebrospinal fluid by mass spectrometry

被引：26

作者：

Burlet-Schiltz, O

Mazarguil, H

Sol, JC

Chaynes, P

Monsarrat, B

Zajac, JM

Roussin, A

机构：

[1] CNRS, UMR 5089, Inst Pharmacol Biol Struct, F-31077 Toulouse, France

[2] CHU Rangueil Larrey, Serv Neurochirurg, F-31403 Toulouse, France

来源：

FEBS LETTERS | 2002年 / 532卷 / 03期

基金：

澳大利亚研究理事会;

关键词：

neuropeptide FF; electrospray tandem mass spectrometry; human cerebrospinal fluid; nanospray ion trap tandem mass spectrometry;

D O I：

10.1016/S0014-5793(02)03686-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several neuropeptide FF (NPFF)-related peptides, known as modulators of the opioid system, have been previously characterized in bovine and rodent brain. Reverse-phase high pressure liquid chromatography (HPLC) fractions of a human with normal pressure hydrocephalus cerebrospinal fluid (CSF), co-migrating with NPFF-related synthetic peptides, were characterized by capillary HPLC coupled on-line to nanospray ion trap tandem mass spectrometry. Two peptides present in the pro-NPFFA precursor, NPAF (AGEGLNSQFWSLAAPQRF-NH2) and NPSF (SLAAPQRF-NH2), were identified. The monitoring of NPFF-related peptides in human CSF can be helpful to understand their roles in pain sensitivity. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：313 / 318

页数：6

共 23 条

[1] AUTORADIOGRAPHIC LOCALIZATION OF RECEPTORS FOR NEUROPEPTIDE FF, FLFQPQRFAMIDE, IN HUMAN SPINAL SENSORY SYSTEM [J].