Europium cryptate labeled deoxyuridine-triphosphate analog: Synthesis and enzymatic incorporation

被引：9

作者：

Alpha-Bazin, B

Bazin, H

Guillemer, S

Sauvaigo, S

Mathis, G

机构：

[1] CIS Bioint, DIVT, Res & New Technol, F-30203 Bagnols, France

[2] CEA Grenoble, SCIB, DRFMC, F-38054 Grenoble 9, France

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2000年 / 19卷 / 09期

关键词：

D O I：

10.1080/15257770008033854

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthesis of an europium tris-bipyridine cryptate labeled 2'-deoxyuridine-5'-triphosphate analog (K-11-dUTP) is described. This labeled triphosphate was incorporated into DNA through enzymatic reactions with terminal transferase and DNA polymerases. The enzymatic reactions were monitored by TRACE (Time Resolved Amplification of Cryptate Emission), a homogeneous method using Fluorescence Resonance Energy Transfer (FRET) from an europium cryptate as donor to a modified allophycocyanine as acceptor.

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页码：1463 / 1474

页数：12

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