Implication of Sphingomyelin/Ceramide Molar Ratio on the Biological Activity of Sphingomyelinase

被引：19

作者：

Boulgaropoulos, Beate ^{[1
]}

Amenitsch, Heinz ^{[1
]}

Laggner, Peter ^{[1
]}

Pabst, Georg ^{[1
]}

机构：

[1] Austrian Acad Sci, Inst Biophys & Nanosyst Res, Graz, Austria

来源：

BIOPHYSICAL JOURNAL | 2010年 / 99卷 / 02期

关键词：

PHOSPHOLIPID MEMBRANE; BACILLUS-CEREUS; LIPID RAFTS; CERAMIDE; CHOLESTEROL; APOPTOSIS; VESICLES; BILAYERS; PHASE; COEXISTENCE;

D O I：

10.1016/j.bpj.2010.04.028

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Sphingolipid signaling plays an important, yet not fully understood, role in diverse aspects of cellular life. Sphingomyelinase is a major enzyme in these signaling pathways, catalyzing hydrolysis of sphingomyelin to ceramide and phosphocholine. To address the related membrane dynamical structural changes and their feedback to enzyme activity, we have studied the effect of enzymatically generated ceramide in situ on the properties of a well-defined lipid model system. We found a gel-phase formation that was about four times faster than ceramide generation due to ceramide-sphingomyelin pairing. The gel-phase formation slowed down when the ceramide molar ratios exceeded those of sphingomyelin and stopped just at the solubility limit of ceramide, due to unfavorable pairwise interactions of ceramide with itself and with monounsaturated phosphatidylcholine. A remarkable correlation to in vitro experiments suggests a regulation of sphingomyelinase activity based on the sphingomyelin/ceramide molar ratio.

引用

页码：499 / 506

页数：8

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