Lactobacillus paracasei normalizes muscle hypercontractility in a murine model of postinfective gut dysfunction

被引:149
作者
Verdú, EF
Bercík, P
Bergonzelli, GE
Huang, XX
Blennerhasset, P
Rochat, F
Fiaux, M
Mansourian, R
Corthésy-Theulaz, I
Collins, SM
机构
[1] McMaster Univ, Intestinal Dis Res Program, Hamilton, ON HSC 3N5C, Canada
[2] Nestle Res Ctr, Dept Nutr & Hlth, Lausanne, Switzerland
基金
加拿大健康研究院;
关键词
D O I
10.1053/j.gastro.2004.06.007
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background & Aims: The effects of probiotics on gut dysfunction in postinfective irritable bowel syndrome are unknown. We tested whether probiotics influence persistent muscle hypercontractility in mice after recovery from infection with Trichinella spiralis and analyzed the underlying mechanisms. Methods: Mice were gavaged with Lactobacillus paracasei, Lactobacillus johnsonii, Bifidobacterium longum, or Bifidobacterium lactis in spent culture medium from days 10 to 21 after infection. Additional mice received heat-inactivated Lactobacillus paracasei, Lactobacillus paracasei-free spent culture medium, or heat-inactivated Lactobacillus paracasei-free spent culture medium. Lactobacilli enumeration, immunohistochemistry, and cytokine detection (enzyme-linked immunosorbent assay) were performed. Mice were also treated with Lactobacillus paracasei or Lactobacillus paracasei-free spent culture medium from days 18 to 28 after infection. Contractility was measured on days 21 and 28 after infection. Results: Lactobacillus paracasei, but not Lactobacillus johnsonii, Bifidobacterium lactis, or Bifidobacterium longum, attenuated muscle hypercontractility. This was associated with a reduction in the Trichinella spiralis-associated T-helper 2 response and a reduction in transforming growth factor-beta1, cyclooxygenase-2, and prostaglandin E-2 levels in muscle. Attenuation of muscle hypercontractility by Lactobacillus paracasei-free spent culture medium was abolished after heat treatment. Improvement of muscle hypercontractility at day 28 after infection was also observed after the administration of Lactobacillus paracasei or Lactobacillus paracasei-free spent culture medium from day 18 after infection. Conclusions: Probiotics show strain-dependent attenuation of muscle hypercontractility in an animal model of postinfective irritable bowel syndrome. This likely occurs via both a modulation of the immunologic response to infection and a direct effect of Lactobacillus paracasei or a heat-labile metabolite on postinfective muscle hypercontractility. Lactobacillus paracasei may be useful in the treatment of postinfective irritable bowel syndrome.
引用
收藏
页码:826 / 837
页数:12
相关论文
共 52 条
[1]  
Akiho H, 2002, GASTROENTEROLOGY, V122, pA256
[2]  
Akiho H, 2002, GASTROENTEROLOGY, V122, pA135
[3]   JEJUNAL MICROBIAL-FLORA OF SOUTHERN INDIAN INFANTS IN HEALTH AND WITH ACUTE GASTROENTERITIS [J].
ALBERT, MJ ;
BHAT, P ;
RAJAN, D ;
MAIYA, PP ;
PEREIRA, SM ;
MATHAN, M ;
BAKER, SJ .
JOURNAL OF MEDICAL MICROBIOLOGY, 1978, 11 (04) :433-440
[4]  
BALSARI A, 1982, MICROBIOLOGICA, V5, P185
[5]   Role of immunologic factors and cyclooxygenase 2 in persistent postinfective enteric muscle dysfunction in mice [J].
Barbara, G ;
De Giorgio, R ;
Deng, YK ;
Vallance, B ;
Blennerhassett, P ;
Collins, SM .
GASTROENTEROLOGY, 2001, 120 (07) :1729-1736
[6]   Persistent intestinal neuromuscular dysfunction after acute nematode infection in mice [J].
Barbara, G ;
Vallance, BA ;
Collins, SM .
GASTROENTEROLOGY, 1997, 113 (04) :1224-1232
[7]  
Bercik P, 2001, GASTROENTEROLOGY, V120, pA71
[8]   CHANGES IN ANORECTAL FUNCTION IN PERSISTENT BOWEL DISTURBANCE FOLLOWING SALMONELLA GASTROENTERITIS [J].
BERGIN, AJ ;
DONNELLY, TC ;
MCKENDRICK, MW ;
READ, NW .
EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY, 1993, 5 (08) :617-620
[9]   INSTABILITY IN THE FECAL FLORA OF A PATIENT SUFFERING FROM FOOD-RELATED IRRITABLE-BOWEL-SYNDROME [J].
BRADLEY, HK ;
WYATT, GM ;
BAYLISS, CE ;
HUNTER, JO .
JOURNAL OF MEDICAL MICROBIOLOGY, 1987, 23 (01) :29-32
[10]   INTERDIGESTIVE MYOELECTRIC COMPLEX IN GERM-FREE RATS [J].
CAENEPEEL, P ;
JANSSENS, J ;
VANTRAPPEN, G ;
EYSSEN, H ;
COREMANS, G .
DIGESTIVE DISEASES AND SCIENCES, 1989, 34 (08) :1180-1184