Endothelium-independent vasorelaxant effect of a Berberis orthobotrys root extract via inhibition of phosphodiesterases in the porcine coronary artery

被引：15

作者：

Alamgeer ^{[1
,2
]}

Chabert, P. ^{[1
]}

Akhtar, M. S. ^{[3
]}

Jabeen, Q. ^{[3
]}

Delecolle, J. ^{[4
]}

Heintz, D. ^{[4
]}

Garo, E. ^{[5
]}

Hamburger, M. ^{[5
]}

Auger, C. ^{[1
]}

Lugnier, C. ^{[1
]}

Kim, H. -J. ^{[6
]}

Oak, M. -H. ^{[6
]}

Schini-Kerth, V. B. ^{[1
]}

机构：

[1] Univ Strasbourg, Fac Pharm, Lab Biophoton & Pharmacol, UMR CNRS 7213, Illkirch Graffenstaden, France

[2] Univ Sargodha, Fac Pharm, Sargodha, Pakistan

[3] Islamia Univ Bahawalpur, Fac Pharm & Alternat Med, Bahawalpur, Pakistan

[4] Univ Strasbourg, IBMP, UPR 2357, Illkirch Graffenstaden, France

[5] Univ Basel, Dept Pharmaceut Sci, CH-4003 Basel, Switzerland

[6] Mokpo Natl Univ, Coll Pharm, Muan Gun 534729, Jeollanamdo, South Korea

来源：

PHYTOMEDICINE | 2016年 / 23卷 / 08期

基金：

新加坡国家研究基金会;

关键词：

Berberis orthobotrys; Vasorelaxation; Endothelium; Phosphodiesterases; CYCLIC-NUCLEOTIDE PHOSPHODIESTERASES; DEPENDENT RELAXATION; PROTEIN-KINASES; RAT AORTA; MODULATION; GMP; NO; CHANNELS; SYSTEM; EDHF;

D O I：

10.1016/j.phymed.2016.04.005

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Background: Berberis orthobotrys Bien ex Aitch. (Berberidaceae) is a plant indigenous of Pakistan that is locally used for the treatment of hypertension. Hypothesis: This study evaluated the vasoactive properties of a Berberis orthobotrys root extract and its fractions, and investigated the role of the endothelium and the underlying mechanism. Study design: An aqueous methanolic extract of Berberis orthobotrys roots was prepared and submitted to a multi-step liquid-liquid fractionation with solvents of increasing polarity. Vascular reactivity of the different fractions was assessed using porcine coronary artery rings either with or without endothelium, and in the presence or absence of specific pharmacological tools. The ability of Berberis orthobotrys extracts to affect phosphodiesterase (PDE) activity was evaluated using a radioenzymatic method and purified phosphodiesterases. Results: The aqueous methanol extract induced similar relaxations in coronary artery rings with and without endothelium, and, amongst the three derived preparations, the butanol fraction (BFBO) was slightly but significantly more effective than the ethyl acetate fraction and the aqueous residue in rings without endothelium. Analysis of the butanol fraction (BFBO) by LC-ELSD-MS indicated the presence of four major isoquinoline alkaloids including berberine. BFBO significantly potentiated the relaxations induced by cyclic GMP- and cyclic AMP-dependent relaxing agonists, and inhibited contractions to KCl, CaCl2, and U46619 in endothelium denuded rings. In contrast, BFBO did not affect relaxations to endothelium-dependent vasodilators. BFBO concentration-dependently inhibited the cyclic GMP-hydrolyzing activity of basal PDE1, calmodulin-activated PDE1 and PDE5, and of cyclic AMP-hydrolyzing activity of PDE3 and PDE4 with IC50 values ranging from 40 to 130 mu g/ml. Conclusion: The butanol fraction of the aqueous methanol extract of Berberis orthobotrys roots induced pronounced endothelium-independent relaxations and inhibited contractile responses by acting directly at the vascular smooth muscle in the coronary artery. Moreover, BFBO potentiated relaxations induced by both cyclic GMP- and cyclic AMP-dependent vasodilators most likely due to its ability to inhibit several vascular PDEs, and in particular PDE4 and PDE5. (C) 2016 Elsevier GmbH. All rights reserved.

引用

页码：793 / 799

页数：7

共 33 条

[1]

Antihypertensive Activity of Aqueous-Methanol Extract of Berberis Orthobotrys Bien Ex Aitch in Rats [J].