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Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice

被引：58
作者：
Qu, D
Zheng, BJ
Yao, X
Guan, Y
Yuan, ZH
Zhong, NS
Lu, LW
Xie, JP
Wen, YM [1 ]
机构：
[1] Fudan Univ, Shanghai Med Coll, Key Lab Med Mol Virol, Minist Educ,Minist Publ Hlth, Shanghai 200032, Peoples R China
[2] Univ Hong Kong, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China
[3] Guangzhou Med Univ, Inst Resp Dis, Guangzhou, Peoples R China
[4] Univ Hong Kong, Dept Pathol, Hong Kong, Hong Kong, Peoples R China
[5] Guangzhou Childrens Hosp, Guangzhou, Peoples R China
来源：
VACCINE | 2005年 / 23卷 / 07期
关键词：
SARS-CoV; intranasal immunization; inactivated vaccine;
D O I：
10.1016/j.vaccine.2004.07.031
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
SARS-CoV (severe acute respiratory syndrome-associated coronavirus) strain GZ50 was partially purified and inactivated with 1:2000 formaldehyde. In cell culture the inactivated virus blocked the replication of live virus by decreasing the TCID5.0 of the live virus 10(3.6) to 10(4.6) times. Inactivated GZ50 was used to immunize mice intranasally either alone, or after precipitation with polyethylene glycol (PEG), or with CpG, or CTB as an adjuvant. The titer of serum neutralizing antibodies was up to 1:640. In mice immunized with adjuvants or PEG precipitated GZ50, specific IgA was detected in tracheal-lung wash fluid by immunofluorescence. Though serum antibodies were detected, no anti-SARS-IgA could be detected in mice immunized only with inactivated GZ50. The roles of adjuvants in intranasal immunization with inactivated. SARS-CoV is discussed. (C) 2004 Elsevier Ltd. All rights reserved.
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收藏
页码：924 / 931
页数：8
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