Accumulation of phosphorylated repressor for gibberellin signaling in an F-box mutant

被引：519

作者：

Sasaki, A

Itoh, H

Gomi, K

Ueguchi-Tanaka, M

Ishiyama, K

Kobayashi, M

Jeong, DH

An, G

Kitano, H

Ashikari, M

Matsuoka, M ^{[1
]}

机构：

[1] Nagoya Univ, Biosci Ctr, Nagoya, Aichi 4648601, Japan

[2] RIKEN, Bioresources Ctr, Tsukuba, Ibaraki 3050074, Japan

[3] Pohang Univ Sci & Technol, Div Mol & Life Sci, Pohang 790784, South Korea

[4] Nagoya Univ, Grad Sch Bioagr Sci, Nagoya, Aichi 4648601, Japan

来源：

SCIENCE | 2003年 / 299卷 / 5614期

关键词：

D O I：

10.1126/science.1081077

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Gibberellin (GA) regulates growth and development in plants. We isolated and characterized a rice GA-insensitive dwarf mutant, gid2. The GID2 gene encodes a putative F-box protein, which interacted with the rice Skp1 homolog in a yeast two-hybrid assay. In gid2, a repressor for GA signaling, SLR1, was highly accumulated in a phosphorylated form and GA increased its concentration, whereas SLR1 was rapidly degraded by GA through ubiquitination in the wild type. We conclude that GID2 is a positive regulator of GA signaling and that regulated degradation of SLR1 is initiated through GA-dependent phosphorylation and finalized by an SCFGID2-proteasome pathway.

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页码：1896 / 1898

页数：3

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