Identification of genes responsible for osteoblast differentiation from human mesodermal progenitor cells

被引:178
作者
Qi, HL
Aguiar, DJ
Williams, SM
La Pean, A
Pan, W
Verfaillie, CM [1 ]
机构
[1] Univ Minnesota, Sch Med, Dept Med, Div Hematol Oncol & Transplantat,Stem Cell Inst, Minneapolis, MN 55445 USA
[2] Univ Minnesota, Sch Publ Hlth, Div Biostat, Minneapolis, MN 55445 USA
关键词
D O I
10.1073/pnas.0532693100
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Single human bone marrow-derived mesodermal progenitor cells (MPCs) differentiate into osteoblasts, chondrocytes, adipocytes, myocytes, and endothelial cells. To identify genes involved in the commitment of MPCs to osteoblasts we examined the expressed gene profile of undifferentiated MPCs and MPCs induced to the osteoblast lineage for 1-7 days by cDNA microarray analysis. As expected, growth factor, hormone, and signaling pathway genes known to be involved in osteogenesis were activated during differentiation. In addition, 41 transcription factors (TFs) were differentially expressed over time, including TFs with known roles in osteoblast differentiation and TFs not known to be involved in osteoblast differentiation. As the latter group of TFs coclustered with osteogenesis-specific TFs, they may play a role in osteoblast differentiation. When we compared the gene expression profile of MPCs induced to differentiate to chondroblasts and osteoblasts, significant differences in the nature and/or timing of gene activation were seen. These studies indicate that in vitro differentiation cultures in which MPCs are induced to one of multiple cell fates should be very useful for defining signals important for lineage-specific differentiation.
引用
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页码:3305 / 3310
页数:6
相关论文
共 38 条
[1]  
Bitzer M, 2000, GENE DEV, V14, P187
[2]   Expression profiling in the muscular dystrophies: Identification of novel aspects of molecular pathophysiology [J].
Chen, YW ;
Zhao, P ;
Borup, R ;
Hoffman, EP .
JOURNAL OF CELL BIOLOGY, 2000, 151 (06) :1321-1336
[3]   The short stature homeobox gene SHOX is involved in skeletal abnormalities in Turner syndrome [J].
Clement-Jones, M ;
Schiller, S ;
Rao, E ;
Blaschke, RJ ;
Zuniga, A ;
Zeller, R ;
Robson, SC ;
Binder, G ;
Glass, I ;
Strachan, T ;
Lindsay, S ;
Rappold, GA .
HUMAN MOLECULAR GENETICS, 2000, 9 (05) :695-702
[4]   Erythroid Kruppel-like factor (EKLF) coordinates erythroid cell proliferation and hemoglobinization in cell lines derived from EKLF null mice [J].
Coghill, E ;
Eccleston, S ;
Fox, V ;
Cerruti, L ;
Brown, C ;
Cunningham, J ;
Jane, S ;
Perkins, A .
BLOOD, 2001, 97 (06) :1861-1868
[5]   The osteoblast: A sophisticated fibroblast under central surveillance [J].
Ducy, P ;
Schinke, T ;
Karsenty, G .
SCIENCE, 2000, 289 (5484) :1501-1504
[6]  
Ducy P, 2000, DEV DYNAM, V219, P461, DOI 10.1002/1097-0177(2000)9999:9999<::AID-DVDY1074>3.0.CO
[7]  
2-C
[8]  
Goltzman D, 2000, CRIT REV EUKAR GENE, V10, P135
[9]   Arrest of G1-S progression by the p53-inducible gene PC3 is Rb dependent and relies on the inhibition of cyclin D1 transcription [J].
Guardavaccaro, D ;
Corrente, G ;
Covone, F ;
Micheli, L ;
D'Agnano, I ;
Starace, G ;
Caruso, M ;
Tirone, F .
MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (05) :1797-1815
[10]   Transplantability and therapeutic effects of bone marrow-derived mesenchymal cells in children with osteogenesis imperfecta [J].
Horwitz, EM ;
Prockop, DJ ;
Fitzpatrick, LA ;
Koo, WWK ;
Gordon, PL ;
Neel, M ;
Sussman, M ;
Orchard, P ;
Marx, JC ;
Pyeritz, RE ;
Brenner, MK .
NATURE MEDICINE, 1999, 5 (03) :309-313