Establishment of an R-banded rabbit karyotype nomenclature by FISH localization of 23 chromosome-specific genes on both G- and R-banded chromosomes

被引:33
作者
Hayes, H [1 ]
Rogel-Gaillard, C
Zijlstra, C
de Haan, NA
Urien, C
Bourgeaux, N
Bertaud, M
Bosma, AA
机构
[1] INRA, Lab Genet Biochim & Cytogenet, F-78352 Jouy En Josas, France
[2] INRA, Lab Radiobiol & Etud Genome, CEA, UMR 13314, F-78352 Jouy En Josas, France
[3] Univ Utrecht, Fac Vet Med, Dept Lab Anim Sci, Utrecht, Netherlands
[4] Univ Utrecht, Fac Vet Med, Dept Biochem Cell Biol & Histol, Utrecht, Netherlands
关键词
D O I
10.1159/000069807
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Direct detection of fluorescent in situ hybridization signals on R-banded chromosomes stained with propidium iodide is a rapid and efficient method for constructing cytogenetic maps for species with R-banded standard karyotypes. In this paper, our aim is to establish an R-banded rabbit karyotype nomenclature that is in total agreement with the 1981 G-banded standard nomenclature. For this purpose, we have produced new GTG- and RBG-banded mid-metaphase karyotypes and an updated version of ideograms of R-banded rabbit chromosomes. In addition, to confirm correlations between G- and R-banded chromosomes, we have defined a set of 23 rabbit BAC clones, each containing a specific gene, one marker gene per rabbit chromosome, and we have localized precisely each BAC clone by FISH on both G- and R-banded chromosomes. Copyright (C) 2002 S. Karger AG, Basel.
引用
收藏
页码:199 / 205
页数:7
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