Characterization of a branched-chain amino-acid aminotransferase from Schizosaccharomyces pombe

被引：1

作者：

Eden, A ^{[1
]}

Benvenisty, N ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Inst Life Sci, Dept Genet, IL-91904 Jerusalem, Israel

来源：

YEAST | 1998年 / 14卷 / 02期

关键词：

branched-chain amino acids; aminotransferase; Schizosaccharomyces pombe; Saccharomyces cerevisiae;

D O I：

10.1002/(SICI)1097-0061(19980130)14:2<189::AID-YEA210>3.0.CO;2-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Saccharomyces cerevisiae genes for the cytosolic and mitochondrial branched-chain amino-acid aminotransferases (BCAT) were isolated recently. These genes show significant homology to mammalian ECA39, originally isolated as st gene regulated by the c-myc oncogene. We now report the isolation of the Schizosaccharomyces pombe eca39/BCAT gene. The S. pombe protein shows 47-52% identity to other eukaryotic BCAT proteins isolated from S. cerevisiae, nematode, mouse and man. A genetic growth assay for BCAT activity was established using an S. cerevisiae strain disrupted in both BCAT isoenzymes. Consequently, the activity of the S. pombe BCAT was demonstrated by genetic and biochemical means. Possible applications of BCAT-encoding genes as selection markers in yeast transformation are proposed. The sequence has been deposited in the GenBank data library under Accession Number U88029. (C) 1998 John Wiley & Sons, Ltd.

引用

页码：189 / 194

页数：6

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