The neuroprotective effect of melatonin against the induction of c-Jun phosphorylation by 6-hydroxydopamine on SK-N-SH cells

被引：24

作者：

Chetsawang, B

Govitrapong, P ^{[1
]}

Ebadi, M

机构：

[1] Mahidol Univ, Inst Sci & Technol Res & Dev, Neurobehav Biol Ctr, Salaya 73170, Nakornpathom, Thailand

[2] Mahidol Univ, Fac Sci, Dept Pharmacol, Ctr Neurosci, Bangkok 10400, Thailand

[3] Univ N Dakota, Sch Med & Hlth Sci, Dept Pharmacol Physiol & Therapeut, Grand Forks, ND 58201 USA

来源：

NEUROSCIENCE LETTERS | 2004年 / 371卷 / 2-3期

关键词：

6-hydroxydopamine; melatonin; c-Jun phosphorylation; neuroprotective; SK-N-SH cells; Parkinson's disease;

D O I：

10.1016/j.neulet.2004.08.068

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Melatonin is synthesized mainly in pineal gland. It has been suggested that melatonin has proven antioxidant effects and protective effects against neuronal cell degeneration. There are several studies indicating that c-Jun-N-terminal kinase pathways might be involved in neuronal cell death. In this study, the effects of melatonin on 6-hydroxydopamine (6-OHDA)-treated cultured SK-N-SH cells were investigated. The results showed that 6-OHDA significantly decreased cell viability as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay, and melatonin was able to reverse the toxic effects of 6-OHDA on cell viability. In addition, induction of c-Jun phosphorylation by 6-OHDA was diminished by melatonin. These results demonstrate some protective properties of melatonin against neuronal cell degeneration and its action on the inhibition of c-Jun-N terminal kinase signaling cascade. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

引用

页码：205 / 208

页数：4

共 21 条

[1] Both oxidative stress-dependent and independent effects of amyloid β protein are detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay [J].