Opening of the extraordinarily stable mini-hairpin d(GCGAAGC)

被引：12

作者：

Jolles, B

Refregiers, W

Laigle, A

机构：

[1] LPBC (CNRS URA 2056), Univ. Pierre et Marie Curie, 75252 Paris Cedex 05

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 22期

关键词：

D O I：

10.1093/nar/25.22.4608

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

For the purposes of the antisense strategy oligodeoxyribonucleotides can be protected against serum and cell nuclease digestion by tagging at their 3'-end with a sequence naturally forming a very stable hairpin, d(GCGAAGC). This nuclease-resistant hairpin is also known for its high thermostability. We demonstrate in this study that attachment of d(GCGAAGC) at the 3'-end of an oligodeoxyribonucleotide does not hinder hybridization of the 5'-part of this oligonucleotide to a complementary DNA strand. Moreover, the hairpin is in equilibrium between a folded and an open structure, with an energy minimum in favor of pairing if it is possible, even with mismatches.

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页码：4608 / 4613

页数：6

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