Surface-chemistry control to silence gene expression in Drosophila Schneider 2 cells through RNA interference

被引：9

作者：

Chan, Eugene W. L.

Yousaf, Muhammad N. ^{[1
]}

机构：

[1] Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA

[2] Univ N Carolina, Carolina Ctr Genome Sci, Chapel Hill, NC 27599 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 21期

关键词：

gene technology; gold; microcontact printing; RNA interference; self-assembled monolayers;

D O I：

10.1002/anie.200604079

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A good combination: RNA interference (RNAi) technology combined with surface chemistry can be used to control RNAi-mediated gene silencing in Drosophila Schneider 2 (S2) cells patterned on tailored self-assembled monolayers of alkanethiolates on gold. The cell population and not the cell confluence was shown to be critical for the effectiveness of RNAi Rho GTPase. (Figure Presented). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：3881 / 3884

页数：4

相关论文

共 16 条

[1] Gene silencing through RNA interference (RNAi) in vivo: Strategies based on the direct application of siRNAs [J].

Aigner, A .

JOURNAL OF BIOTECHNOLOGY, 2006, 124 (01) :12-25

[2] Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways [J].

Clemens, JC ;

Worby, CA ;

Simonson-Leff, N ;

Muda, M ;

Maehama, T ;

Hemmings, BA ;

Dixon, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6499-6503

[3] Rho GTPases in cell biology [J].

Etienne-Manneville, S ;

Hall, A .

NATURE, 2002, 420 (6916) :629-635

[4] Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].

Fire, A ;

Xu, SQ ;

Montgomery, MK ;

Kostas, SA ;

Driver, SE ;

Mello, CC .

NATURE, 1998, 391 (6669) :806-811

[5]

Gilmore Ian R., 2006, Current Drug Delivery, V3, P147, DOI 10.2174/156720106776359159

[6] An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells [J].

Hammond, SM ;

Bernstein, E ;

Beach, D ;

Hannon, GJ .

NATURE, 2000, 404 (6775) :293-296

[7] RNAi-mediated pathways in the nucleus [J].

Matzke, MA ;

Birchler, JA .

NATURE REVIEWS GENETICS, 2005, 6 (01) :24-35

[8] Mechanisms of gene silencing by double-stranded RNA [J].

Meister, G ;

Tuschl, T .

NATURE, 2004, 431 (7006) :343-349

[9]

Morris KV, 2006, CURR OPIN MOL THER, V8, P115

[10] Molecular requirements for actin-based lamella formation in Drosophila S2 cells [J].

Rogers, SL ;

Wiedemann, U ;

Stuurman, N ;

Vale, RD .

JOURNAL OF CELL BIOLOGY, 2003, 162 (06) :1079-1088