Short- and long-term effects of perinatal asphyxia on monoamine, amino acid and glycolysis product levels measured in the basal ganglia of the rat

被引:57
作者
Chen, Y
Engidawork, E
Loidl, F
Dell'Anna, E
Goiny, M
Lubec, G
Andersson, K
Herrera-Marschitz, M [1 ]
机构
[1] Karolinska Inst, Dept Physiol & Pharmacol, S-17177 Stockholm, Sweden
[2] Karolinska Inst, Dept Internal Med, S-17177 Stockholm, Sweden
[3] Univ Udine, Neurol Inst, Dept Expt & Clin Pathol & Med, I-33100 Udine, Italy
[4] Univ Vienna, Dept Paediat, A-1090 Vienna, Austria
来源
DEVELOPMENTAL BRAIN RESEARCH | 1997年 / 104卷 / 1-2期
关键词
perinatal asphyxia; dopamine; aspartate; glutamate; neostriatum; substantia nigra; ventral tegmental area; nucleus accumbens; rat;
D O I
10.1016/S0165-3806(97)00131-4
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The effects of perinatal asphyxia on levels of dopamine (DA) and its metabolites, amino acids and glycolysis products, measured in tissue samples from substantia nigra(SN), striatum, ventral tegmental area (VTA), and nucleus accumbens (Acb), were studied 80 min to 8 days after birth with high performance liquid chromatography (HPLC). Furthermore, extracellular levels of DA, amino acids and glycolysis products were measured with in vivo microdialysis in the striatum 40-140 min and 4 weeks after birth. Asphyxia was induced by immersing foetus-containing uterus horns, removed from ready-to-deliver Sprague-Dawley rats, in a water bath at 37 degrees C for various time periods (0-22 min). Spontaneous-and caesarean-delivered pups were used as controls. Perinatal asphyxia led to a decrease in the rate of survival, depending upon the length of the insult. In parallel, lactate (LACT) levels were increased with the length of the insult in all examined brain regions, monitored ex vivo or in vivo immediately after birth, DA, glutamate (GLU) and aspartate (ASP) levels were also increased, mainly in tissue samples taken from the mesencephalon. Only minor changes were observed in tissue samples taken from the telencephalon. However, in experiments with in vivo microdialysis, DA and GLU levels were increased following 20-21 and 21-22 min of perinatal asphyxia, but the effect of K+ depolarisation on extracellular DA and ASP levels was strongly diminished. DA and metabolites increased with development in SN and striatum, with no clear differences between control and asphyctic rats. However, 8 days after birth, it was found that DA levels were increased, alternatively decreased in mesencephalic and telencephalic regions following 20-21 and 21-22 min of perinatal asphyxia, periods associated with 60% and 90% of perinatal mortality, respectively. Furthermore, in microdialysis experiments performed 4 weeks after birth, extracellular DA and its metabolites levels were also increased, alternatively decreased in rats exposed to a 20-21 and 21-22 min perinatal asphyctic insult. In this last group, GLU and ASP levels were also decreased. Furthermore, the effect of K+ depolarisation on DA and ASP levels was strongly decreased in both asphyctic groups. Thus, perinatal asphyxia produces short-and long-term consequences in general metabolism, and induces region-specific changes in several neurotransmitter systems, mainly affecting meso-telencephalic DA systems. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:19 / 30
页数:12
相关论文
共 37 条
[1]   PERINATAL ASPHYXIA INCREASES BFGF MESSENGER-RNA LEVELS AND DA CELL BODY NUMBER IN THE MESENCEPHALON OF RATS [J].
ANDERSSON, K ;
BLUM, M ;
CHEN, Y ;
ENEROTH, P ;
GROSS, J ;
HERRERAMARSCHITZ, M ;
BJELKE, B ;
BOLME, P ;
DIAZ, R ;
JAMISON, L ;
LOIDL, F ;
UNGETHUM, U ;
ASTROM, G ;
OGREN, SO .
NEUROREPORT, 1995, 6 (02) :375-378
[2]  
ANDERSSON K, 1992, HYPOXIA ISCHEMIA, V41, P71
[3]  
Beazley JM, 1989, OBSTETRICS, P857
[4]   ASPHYCTIC LESION - PROLIFERATION OF TYROSINE HYDROXYLASE-IMMUNOREACTIVE NERVE-CELL BODIES IN THE RAT SUBSTANTIA-NIGRA AND FUNCTIONAL-CHANGES IN DOPAMINE NEUROTRANSMISSION [J].
BJELKE, B ;
ANDERSSON, K ;
OGREN, SO ;
BOLME, P .
BRAIN RESEARCH, 1991, 543 (01) :1-9
[5]   NICOTINE TREATMENT COUNTERACTS PERINATAL ASPHYXIA-INDUCED CHANGES IN THE MESOSTRIATAL LIMBIC DOPAMINE SYSTEMS AND IN MOTOR BEHAVIOR IN THE 4-WEEK-OLD MALE-RAT [J].
CHEN, Y ;
OGREN, SO ;
BJELKE, B ;
BOLME, P ;
ENEROTH, P ;
GROSS, J ;
LOIDL, F ;
HERRERAMARSCHITZ, M ;
ANDERSSON, K .
NEUROSCIENCE, 1995, 68 (02) :531-538
[6]   Perinatal asphyxia-induced changes in rat brain tyrosine hydroxylase-immunoreactive cell body number: Effects of nicotine treatment [J].
Chen, Y ;
HerreraMarschitz, M ;
Bjelke, B ;
Blum, M ;
Gross, J ;
Andersson, K .
NEUROSCIENCE LETTERS, 1997, 221 (2-3) :77-80
[7]   Postnatal development of the dopamine transporter: A quantitative autoradiographic study [J].
Coulter, CL ;
Happe, HK ;
Murrin, LC .
DEVELOPMENTAL BRAIN RESEARCH, 1996, 92 (02) :172-181
[8]  
DAHLSTROM A, 1964, ACTA PHYSIOL SCAND, V62, P1
[9]   Delayed neuronal death following perinatal asphyxia in rat [J].
DellAnna, E ;
Chen, Y ;
Engidawork, E ;
Andersson, K ;
Lubec, G ;
Luthman, J ;
HerreraMarschitz, M .
EXPERIMENTAL BRAIN RESEARCH, 1997, 115 (01) :105-115
[10]   SHORT-TERM EFFECTS OF PERINATAL ASPHYXIA STUDIED WITH FOS-IMMUNOCYTOCHEMISTRY AND IN-VIVO MICRODIALYSIS IN THE RAT [J].
DELLANNA, E ;
CHEN, Y ;
LOIDL, F ;
ANDERSSON, K ;
LUTHMAN, J ;
GOINY, M ;
RAWAL, R ;
LINDGREN, T ;
HERRERAMARSCHITZ, M .
EXPERIMENTAL NEUROLOGY, 1995, 131 (02) :279-287