Quantitative study of competitive binding of drugs to protein by microdialysis high performance liquid chromatography

被引：53

作者：

Wang, HL ^{[1
]}

Zou, HF ^{[1
]}

Zhang, YK ^{[1
]}

机构：

[1] Acad Sinica, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 1998年 / 70卷 / 02期

关键词：

D O I：

10.1021/ac970564u

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A displacement equation describing competitive binding of drugs to protein in solution is derived and examined with four nonsteroidal anti-inflammatory drugs and human serum albumin as model drugs and protein, respectively. Microdialysis/high-performance liquid chromatography was adopted to determine simultaneously the unbound solute and displacing agent in drug-protein solutions. The method is able to locate the binding site and determine affinity constants even up to 10(7) L/mol accurately. A comparison of association constants determined by this method and from capacity factors on HSA-CSP is given.

引用

页码：373 / 377

页数：5

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