Isolation and characterization of polymorphic microsatellite loci in nurse shark (Ginglymostoma cirratum)

被引：19

作者：

Heist, EJ ^{[1
]}

Jenkot, JL ^{[1
]}

Keeney, DB ^{[1
]}

Lane, RL ^{[1
]}

Moyer, GR ^{[1
]}

Reading, BJ ^{[1
]}

Smith, NL ^{[1
]}

机构：

[1] So Illinois Univ, Dept Zool, Fisheries & Illinois Aquaculture Ctr, Carbondale, IL 62901 USA

来源：

MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 01期

关键词：

breeding structure; Ginglymostoma cirratum; microsatellite; nurse shark;

D O I：

10.1046/j.1471-8286.2003.00348.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report on the development of nine polymorphic microsatellite loci in nurse shark (Ginglymostoma cirratum) using a combination of enriched and unenriched subgenomic libraries. Based on the small percentage of positive clones in the unenriched library (0.4%) it appears that microsatellites are very scarce in nurse shark genomes. Numbers of alleles at polymorphic loci ranged from two to 15; observed heterozygosity ranged from 0.17 to 0.90. We expect these loci to be useful for studies of breeding structure and paternity.

引用

页码：59 / 61

页数：3

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