Characterization of the inhabitancy of mouse intestinal bacteria (MIB) in rodents and humans by real-time PCR with group-specific primers

被引：13

作者：

Kibe, Ryoko

Sakamoto, Mitsuo

Yokota, Hiroshi

Benno, Yoshimi

机构：

[1] RIKEN Bioresource Ctr, Microbe Div Japan Collect Microorganisms, Wako, Saitama 3510198, Japan

[2] Rakuno Gakuen Univ, Sch Vet Med, Dept Vet Biochem, Ebetsu, Hokkaido 0698501, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2007年 / 51卷 / 04期

关键词：

mouse intestinal bacteria (MIB); group-specific primers; real-time PCR;

D O I：

10.1111/j.1348-0421.2007.tb03916.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Mouse intestinal bacteria (MIB) is a new operational taxonomic unit (OTU) belonging to the Bacteroides subgroup in the Cytophaga-Flavobacter-Bacteroides (CFB) phylum recently found in the intestine of mice, rats and humans. However, their characters are still unknown since they have not yet been isolated by culture. To understand their habitat characteristics in intestinal tracts, the quantification assays of MIB were established using MIB group-specific primers. The MIB population in the intestine was evaluated as a percentage of the number of 16S rRNA gene copy of MIB. A real-time PCR assay using group-specific primers showed the fluctuation of MIB inhabitancy and revealed that the NUB population in the small intestine of mice was significantly lower than the large intestinal contents. Moreover, MIB was found in human feces though the number was lower than in murine. This assay using group-specific primers revealed new information about host-preference of MIB.

引用

页码：349 / 357

页数：9

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