Development and evaluation of a dipstick assay for detection of Plasmodium falciparum and P-vivax sporozoites in mosquitoes (Diptera: Culicidae)

被引：10

作者：

Coleman, RE ^{[1
]}

Barth, JF ^{[1
]}

Turell, MJ ^{[1
]}

Gordon, SW ^{[1
]}

Sattabongkot, J ^{[1
]}

Copeland, R ^{[1
]}

Wirtz, RA ^{[1
]}

机构：

[1] USA, Med Res Inst Infect Dis, Div Virol, Dept Vector Assessment, Ft Detrick, MD 21702 USA

来源：

JOURNAL OF MEDICAL ENTOMOLOGY | 2000年 / 37卷 / 04期

关键词：

detection; dipstick; circumsporozoite protein; mosquito; malaria;

D O I：

10.1603/0022-2585-37.4.581

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

We developed a nitrocellulose-based, dipstick circumsporozoite (CS)-enzyme immunoassay [ELISA] for the simultaneous detection of Plasmodium falciparum and P. vivax-210 CS protein. The assay had a detection threshold of <250 P. falciparum or 400 P. vivax sporozoites per sample, gave results concordant with dissection of salivary glands and CS-ELISA, but was slightly less sensitive than the CS-ELISA in microtiter plates. The assay consistently detected one infected mosquito in a pool of 10 or 20 mosquitoes, and was 100% specific in discriminating between species of Plasmodium when mosquito suspensions were spiked with sporozoites. The assay could be completed in 1 h, required no specialized equipment, and therefore was useful for field applications.

引用

页码：581 / 587

页数：7

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