An antisense chalcone synthase cDNA leads to novel colour patterns in lisianthus (Eustoma grandiflorum) flowers

被引:68
作者
Deroles, SC
Bradley, JM
Schwinn, KE
Markham, KR
Bloor, S
Manson, DG
Davies, KM
机构
[1] New Zealand Inst Crop & Food Res Ltd, Levin Res Ctr, Levin, New Zealand
[2] Ind Res Ltd, Lower Hutt, New Zealand
关键词
antisense; chalcone synthase; flower colour; lisianthus; transformation;
D O I
10.1023/A:1009621903402
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Three cultivars of lisianthus (Eustoma grandiflorum (Grise.)) were transformed with a homologous antisense CHS cDNA via Agrobacterium-mediated transformation. Over 50% of the transgenics derived from the purple flowering lines exhibited an altered flower colour pattern ranging from small streaks of white on the wild-type purple background through to completely white flowers. A significant portion of the transgenic lines showed unstable phenotypes. Northern and biochemical analysis showed that the altered flower patterns were associated with a loss of CHS gene transcript and a corresponding loss of CHS enzyme activity. In the white flowering line the level of total flavonoids was reduced to ca. 2.0% of the wild-type level. Some of the transgenic plants also exhibited alterations in flower form such as the formation of frilled petal tips and reduced flower opening. Several of the new patterned lines ale being evaluated for stability and possible commercial release.
引用
收藏
页码:59 / 66
页数:8
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