Retroviral vector targeting to melanoma cells by single-chain antibody incorporation in envelope

被引：50

作者：

Martin, F

Kupsch, J

Takeuchi, Y

Russell, S

Cosset, FL

Collins, M

机构：

[1] Inst Canc Res, Chester Beatty Labs, CRC, Ctr Cell & Mol Biol, London W1P 6DB, England

[2] Wellcome Res Labs, Beckenham BR3 3B5, Kent, England

[3] Cambridge Ctr Prot Engn, Cambridge CB2 2HK, England

[4] Univ Lyon 1, Ctr Genet Mol & Cellulaire, CNRS UMR5534, F-69622 Villeurbanne, France

来源：

HUMAN GENE THERAPY | 1998年 / 9卷 / 05期

基金：

英国惠康基金;

关键词：

D O I：

10.1089/hum.1998.9.5-737

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two strategies for targeting recombinant retroviruses to melanoma cells were compared, One was to extend the tropism of an ecotropic envelope to human melanoma cells, the other was to enhance the tropism of an amphotropic envelope for melanoma cells, Chimeric retroviral envelopes, incorporating a single-chain antibody (ScFv) directed against high-molecular-weight melanoma-associated antigen (HMWMAA) at the amino terminus are correctly processed and incorporated into virions, ScFv-ecotropic envelope chimeras allow specific, but low-titer, targeting of HMWMAA-positive cells, when co-expressed with ecotropic envelopes, ScFv-amphotropic envelope chimeras bind specifically to HMWMAA-positive cells and allow preferential infection at high titer.

引用

页码：737 / 746

页数：10

共 46 条

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