Capillary electrophoretic separation of glycoproteins

被引:3
作者
Chen, Y [1 ]
机构
[1] Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
来源
SCIENCE IN CHINA SERIES B-CHEMISTRY | 1998年 / 41卷 / 01期
基金
中国国家自然科学基金;
关键词
glycoprotein; microheterogeneity; capillary electrophoresis;
D O I
10.1007/BF02875560
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A capillary electrophoretic approach has been developed for analyzing the microheterogeneity of glycoproleins. The key factors include the buffer composition, concentration, pH, and the surface treatment of the capillaries used. It has been shown that, if the buffer is mainly composed of alcohol amines and the capillaries are coated with polyacrylamide, ovalbumin from chicken egg can be separated into more than 25 peaks and that of transferrin about 10 peaks.
引用
收藏
页码:71 / 76
页数:6
相关论文
共 9 条
[1]  
CHEN Y, 1994, BIOCH TECHNOLOGY GLY, P116
[2]   APPLICATION OF FREE-SOLUTION CAPILLARY ELECTROPHORESIS TO THE ANALYTICAL SCALE SEPARATION OF PROTEINS AND PEPTIDES [J].
GROSSMAN, PD ;
COLBURN, JC ;
LAUER, HH ;
NIELSEN, RG ;
RIGGIN, RM ;
SITTAMPALAM, GS ;
RICKARD, EC .
ANALYTICAL CHEMISTRY, 1989, 61 (11) :1186-1194
[3]   SEPARATION OF THE HUMAN TRANSFERRIN ISOFORMS BY CARRIER-FREE HIGH-PERFORMANCE ZONE ELECTROPHORESIS AND ISOELECTRIC-FOCUSING [J].
KILAR, F ;
HJERTEN, S .
JOURNAL OF CHROMATOGRAPHY, 1989, 480 :351-357
[4]   HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS OF GLYCOPROTEINS - THE USE OF MODIFIERS OF ELECTROOSMOTIC FLOW FOR ANALYSIS OF MICROHETEROGENEITY [J].
LANDERS, JP ;
ODA, RP ;
MADDEN, BJ ;
SPELSBERG, TC .
ANALYTICAL BIOCHEMISTRY, 1992, 205 (01) :115-124
[5]   ANALYSIS OF THE MICROHETEROGENEITY OF THE GLYCOPROTEIN CHORIONIC-GONADOTROPIN WITH HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS [J].
MORBECK, DE ;
MADDEN, BJ ;
MCCORMICK, DJ .
JOURNAL OF CHROMATOGRAPHY A, 1994, 680 (01) :217-224
[6]   ALPHA,OMEGA-BIS-QUATERNARY AMMONIUM ALKANES AS EFFECTIVE BUFFER ADDITIVES FOR ENHANCED CAPILLARY ELECTROPHORETIC SEPARATION OF GLYCOPROTEINS [J].
ODA, RP ;
MADDEN, BJ ;
SPELSBERG, TC ;
LANDERS, JP .
JOURNAL OF CHROMATOGRAPHY A, 1994, 680 (01) :85-92
[7]   PRIMARY STRUCTURE AND FUNCTION OF NOVEL O-GLYCOSYLATED HIRUDINS FROM THE LEECH HIRUDINARIA-MANILLENSIS [J].
STEINER, V ;
KNECHT, R ;
BORNSEN, KO ;
GASSMANN, E ;
STONE, SR ;
RASCHDORF, F ;
SCHLAEPPI, JM ;
MASCHLER, R .
BIOCHEMISTRY, 1992, 31 (08) :2294-2298
[8]   SEPARATION OF CARBOHYDRATE-MEDIATED MICROHETEROGENEITY OF RECOMBINANT-HUMAN-ERYTHROPOIETIN BY FREE SOLUTION CAPILLARY ELECTROPHORESIS - EFFECTS OF PH, BUFFER TYPE AND ORGANIC ADDITIVES [J].
TRAN, AD ;
PARK, SG ;
LISI, PJ ;
HUYNH, OT ;
RYALL, RR ;
LANE, PA .
JOURNAL OF CHROMATOGRAPHY, 1991, 542 (02) :459-471
[9]   CAPILLARY ELECTROPHORETIC SEPARATION OF HUMAN RECOMBINANT ERYTHROPOIETIN (RHUEPO) GLYCOFORMS [J].
WATSON, E ;
YAO, F .
ANALYTICAL BIOCHEMISTRY, 1993, 210 (02) :389-393