A modular diffuse reflection and fluorescence emission imaging colorimeter for the in vivo study of parameters related with the phototoxic effect in PDT.

被引：3

作者：

Balas, CJ ^{[1
]}

Stefanidou, MP ^{[1
]}

Giannouli, TC ^{[1
]}

Georgiou, SK ^{[1
]}

Helidonis, ES ^{[1
]}

Tosca, AD ^{[1
]}

机构：

[1] FORTH, LESL, Laser & Applicat Div, Crete, Greece

来源：

PHOTOCHEMOTHERAPY: PHOTODYNAMIC THERAPY AND OTHER MODALITIES III, PROCEEDINGS OF | 1997年 / 3191卷

关键词：

photodynamic therapy; fluorescence imaging; colorimetry;

D O I：

10.1117/12.297830

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The biological response to PDT depends on the photosensitizer accumulation to the malignant cells, the tumor vasculature, the inherent cell sensitivity to the photodynamic effect etc. The in-vivo measurement of physical parameters related with these factors can serve in the understanding of the mechanisms involved in the cell death, as well as in the optimization of the treatment procedure. In this paper we demonstrate a modular diffuse reflection and fluorescence emission imaging colorimeter. We have use it for the in-vivo on-line quantitative measurement and mapping the time course fluorescence intensity after ALA application and for the tissue color changes associated with the erythema developed during irradiation. Based on the obtained quantitative data, we investigate the correlation between the photosensitizer accumulation before light irradiation and the haemodynamic changes, revealed as erythema development, occurring during light irradiation. Their relative significance as predicting factors for the photodynamic treatment effectiveness is also evaluated.

引用

页码：50 / 57

页数：8