Linker-mediated modulation of the chemiluminescent signal from N10-(3-sulfopropyl)-N-sulfonylacridinium-9-carboxamide tracers

被引：12

作者：

Adamczyk, M ^{[1
]}

Chen, YY ^{[1
]}

Fishpaugh, JR ^{[1
]}

Mattingly, PG ^{[1
]}

Pan, Y ^{[1
]}

Shreder, K ^{[1
]}

Yu, ZG ^{[1
]}

机构：

[1] Abbott Labs, Div Diagnost, Dept Chem D9NM, Abbott Pk, IL 60064 USA

来源：

BIOCONJUGATE CHEMISTRY | 2000年 / 11卷 / 05期

关键词：

D O I：

10.1021/bc0000280

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Four chemiluminescent N-sulfonylacridinium-9-carboxamide active esters (17-20) were prepared from the corresponding acids and coupled to bath of the aminated phenobarbital (13) and N-(6-aminohexyl)phenytoin (16) haptens. The level of signal produced by chemiluminescent N- sulfonylacridinium-9-carboxamide phenobarbital and phenytoin tracers in a solid-phase immunoassay format was found to be modulated by at least 20-fold by the judicious choice of the reactive acridinium-hapten linking group.

引用

页码：714 / 724

页数：11

共 36 条

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