Affinity extraction of lactate dehydrogenase by aqueous two-phase systems using free triazine dyes

被引：10

作者：

Lin, DQ

Zhu, ZQ

Mei, LH

机构：

[1] Dept. of Chem. Eng., Zhcjiang University, Hangzhou

来源：

BIOTECHNOLOGY TECHNIQUES | 1996年 / 10卷 / 01期

关键词：

D O I：

10.1007/BF00161082

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Affinity partitioning of lactate dehydrogenase (LDH) was studied in polyethylene glycol (PEG)/salt and PEG/hydroxypropyl starch (PES) aqueous two-phase systems, using free triazine dyes as their affinity ligands. The free dyes showed one-sided partition to the top PEG-rich phase and thus enhanced the affinity partitioning effect in the systems. A two-step affinity extraction process has been discussed for large scale purification of LDH from rabbit muscle.

引用

页码：41 / 46

页数：6

共 9 条

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