Relationships between toxin gene content and genetic background in nasal carried isolates of Staphylococcus aureus from Asturias, Spain

被引：26

作者：

Fueyo, JM

Mendoza, MC

Alvarez, MA

Martín, MC

机构：

[1] Univ Oviedo, Fac Med, Area Microbiol, Dept Biol Func, Oviedo 33006, Spain

[2] CSIC, Inst Prod Lacteos Asturias, Villaviciosa, Asturias, Spain

来源：

FEMS MICROBIOLOGY LETTERS | 2005年 / 243卷 / 02期

关键词：

Staphylococcus aureus; enterotoxins; superantigens; PCR; PFGE;

D O I：

10.1016/j.femsle.2005.01.006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Staphylococcus aureus recovered from nasal carriers, producers and non-producers (43 isolates each) of classical pyrogenic toxin superantigens (PTSAgs), were screened for 17 additional PTSAg-genes by PCR. Percentages of 88.4 and 65.1 were positive for some new en terotoxin-gene, and 76.7 and 55.8 for entero toxin-gene-clusters (egc-like), respectively. The 86 isolates belonged to 17 toxin-genotypes (all eta-, etb-, etd-, see- and sep-negative), and generated 40 SmaI-genomic profiles that in a dendrogram of similarity (Sgreater than or equal to0.7) clustered into nine lineages and 11 non-clustered branches. Correlations between classical PTSAgs and SmaI-lineages were established and egc-like groupings appeared dispersed in six lineages. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

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页码：447 / 454

页数：8

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