Ectopic expression of the human adenine nucleotide translocase, isoform 3 (ANT-3). Characterization of ligand binding properties

被引：13

作者：

Carroll, AK ^{[1
]}

Clevenger, WR ^{[1
]}

Szabo, T ^{[1
]}

Ackermann, LE ^{[1
]}

Pei, Y ^{[1
]}

Ghosh, SS ^{[1
]}

Glasco, S ^{[1
]}

Nazarbaghi, R ^{[1
]}

Davis, RE ^{[1
]}

Anderson, CM ^{[1
]}

机构：

[1] MitoKor Inc, San Diego, CA 92121 USA

来源：

MITOCHONDRION | 2005年 / 5卷 / 01期

关键词：

adenine nucleotide translocase; ligand; bongkrekic acid; atractyloside;

D O I：

10.1016/j.mito.2004.06.004

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The adenine nucleotide translocase (ANT) is a key component in maintaining cellular energy homeostasis, and has also been implicated in formation of the mitochondrial permeability transition pore. Human ANT-3 was cloned from a human heart cDNA library and expressed as a histidine-tagged fusion protein in the mitochondria of the Trichoplusia ni. cell line. Overexpression resulted in a concomitant decrease in the endogenous ANT content, allowing for the characterization of binding of known ANT ligands to the human protein. Binding affinities for bongkrekic acid (BKA), ADP, and atractyloside (ATR) were measured in mitochondria from the human ANT-3 expressing cell line, and compared to similar preparations from bovine heart mitochondria by use of a novel radioiodinated derivative of ATR. Binding to ANT-3 by the high affinity inhibitors BKA and ATR, as well as the lower affinity natural ligand ADP, was similar to that measured in bovine heart mitochondria, and to that previously reported for mammalian heart mitochondria. Characterizations such as these of human ANT isoforms may lead to drug development for enhanced mitochondrial function and cellular viability. (C) 2004 Published by Elsevier B.V. on behalf of Mitochondria Research Society.

引用

页码：1 / 13

页数：13

共 44 条

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