An Automated MagStation for High-Throughput Single Nucleotide Polymorphism Genotyping and the Dual-Color Hybridization

被引：122

作者：

Tian, Lan ^{[1
]}

Li, Song ^{[1
,2
]}

Liu, Hongna ^{[1
]}

Wang, Zhifei ^{[3
]}

He, Nongyue ^{[1
,2
]}

机构：

[1] Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Peoples R China

[2] Hunan Univ Technol, Hunan Key Lab Green Packaging & Applicat Biol Nan, Zhuzhou 412008, Peoples R China

[3] Southeast Univ, Sch Chem & Chem Engn, Nanjing 210096, Peoples R China

来源：

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | 2009年 / 5卷 / 05期

关键词：

Magnetic Nanoparticles; Automated Magstation; Dual-Color Fluorescent Detection; High-Throughput; Single Nucleotide Polymorphism; Bioelectronics; MAGNETIC NANOPARTICLES; SYSTEM; GENES; PCR;

D O I：

10.1166/jbn.2009.1066

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

We designed a novel automated MagStation for high-throughput single nucleotide polymorphism (SNP) discrimination using dual-color hybridization. Magnetic particles (MPs) were used as target DNA carriers. The principle for SNP discrimination in this study was allele specific hybridization using dual-color fluorescent (Cy3, Cy5) probes. The MagStation is equipped with a 96-well automated magnetic separation unit which collects MPs on the side of the tubes as it moves to separation positions. The MagStation equips a heating block, where hybridization at different optimized temperature can be performed. This MagStation permits heating and magnetic separation of 96 samples per assay. The methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphism of 96 different samples was semi-automated integrated using this automated MagStation.

引用

页码：511 / 515

页数：5

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