Cloning and physical mapping of the EcoRI fragments of the giant linear plasmid SCP1

被引：24

作者：

Redenbach, M

Ikeda, K

Yamasaki, M

Kinashi, H ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Engn, Dept Mol Biotechnol, Higashihiroshima 7398527, Japan

[2] Univ Kaiserslautern, Dept Genet, Genome Res Unit, D-67653 Kaiserslautern, Germany

来源：

JOURNAL OF BACTERIOLOGY | 1998年 / 180卷 / 10期

关键词：

D O I：

10.1128/JB.180.10.2796-2799.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A cosmid library was constructed for the 350-kb giant linear plasmid SCP1 and aligned on a successive linear map. Only a 0.8-kb gap has remained uncloned in the terminal inverted repeats close to both ends. Partial digestion of the aligned cosmids with EcoRI and hybridization with the flanking fragments of the vector enabled physical mapping of all of the EcoRI fragments. On this map, the methylenomycin biosynthetic gene cluster, the insertion sequence IS466, and the sapCDE genes coding for spore-associated proteins were localized.

引用

页码：2796 / 2799

页数：4

共 30 条

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